肠道渗透性生物标志物与代谢健康肥胖症中的炎症有关,但与正常体重肥胖症无关。

IF 4.1 2区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS American journal of physiology. Heart and circulatory physiology Pub Date : 2024-11-01 Epub Date: 2024-08-30 DOI:10.1152/ajpheart.00381.2024
Bryant H Keirns, Austin R Medlin, Katherine A Maki, Kristen McClanahan, Sarah E Fruit, Christina M Sciarrillo, Samantha M Hart, Jill Joyce, Edralin A Lucas, Sam R Emerson
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引用次数: 0

摘要

据报道,正常体重肥胖症(NWO)和代谢健康肥胖症(MHO)都存在全身性炎症,这可能与他们患心血管疾病(CVD)的风险增加有关。然而,这种炎症的驱动因素仍不清楚。我们研究了 NWO 和 MHO、健康对照(CON)和代谢不健康肥胖(MUO;N=80;n=20/组)中已知的影响炎症状态的因素(即肠道通透性、脂肪组织、饮食质量、微生物群)及其与测量的炎症之间的关系。肠道渗透性和炎症的血清指标采用 ELISA 和/或多重方法进行评估。采用双能 X 射线吸收测定法(DXA)测量总脂肪、内脏脂肪和身体脂肪百分比。粪便微生物群组成通过 16S rRNA 测序进行评估(9-10 人/组)。C反应蛋白(CRP)方面,MUO > NWO > CON(pp'sp'spp = 0.11)。与 MUO 相比,CON 的香农指数更高(pp'sp'spp = 0.11)。
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Biomarkers of intestinal permeability are associated with inflammation in metabolically healthy obesity but not normal-weight obesity.

Systemic inflammation is reported in normal-weight obesity (NWO) and metabolically healthy obesity (MHO), which may be linked to their increased cardiovascular disease (CVD) risk. Yet, drivers of this inflammation remain unclear. We characterized factors known to influence inflammatory status (i.e., intestinal permeability, adipose tissue, diet quality, microbiota), and their relationships with measured inflammation, in NWO and MHO, healthy control subjects (CON), and metabolically unhealthy obesity (MUO; N = 80; n = 20/group). Serum indicators of intestinal permeability and inflammation were assessed by ELISA and/or multiplex. Total, visceral, and percent body fat were measured with dual-energy X-ray absorptiometry (DXA). Fecal microbiota composition was assessed via 16S rRNA sequencing (n = 9-10/group). For C-reactive protein (CRP), MUO > NWO > CON (P < 0.0001). In MHO, CRP was intermediate and similar to both MUO and NWO. Lipopolysaccharide binding protein (LBP) and the ratio of LBP to soluble CD14 (sCD14) were higher in MHO and MUO vs. CON/NWO (P < 0.0001). Across correlation and regression analyses, LBP consistently displayed the strongest relationships with CRP in the entire sample (r = 0.78; β = 0.57; P < 0.0001) and in MHO (r = 0.74; P < 0.01) but not NWO (r = 0.37; P = 0.11). Shannon index was higher in CON compared with MUO (P < 0.05) and inversely correlated with CRP in the full sample (r = -0.37; P < 0.05). These data are consistent with the notion that intestinal permeability is associated with low-grade inflammation in MHO, which could be implicated in this population's reported CVD risk.NEW & NOTEWORTHY This is the first study to our knowledge to examine biomarkers of intestinal permeability in normal-weight obesity and one of few assessing microbiota compositions in this population. Additionally, we report that individuals with metabolically healthy obesity and metabolically unhealthy obesity displayed similar evidence of intestinal permeability, which was more strongly associated with systemic inflammation than total and visceral adipose tissue mass.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
202
审稿时长
2-4 weeks
期刊介绍: The American Journal of Physiology-Heart and Circulatory Physiology publishes original investigations, reviews and perspectives on the physiology of the heart, vasculature, and lymphatics. These articles include experimental and theoretical studies of cardiovascular function at all levels of organization ranging from the intact and integrative animal and organ function to the cellular, subcellular, and molecular levels. The journal embraces new descriptions of these functions and their control systems, as well as their basis in biochemistry, biophysics, genetics, and cell biology. Preference is given to research that provides significant new mechanistic physiological insights that determine the performance of the normal and abnormal heart and circulation.
期刊最新文献
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