细胞壁多糖在应对油菜根涝胁迫中的作用

IF 4.4 1区 生物学 Q1 BIOLOGY BMC Biology Pub Date : 2024-09-02 DOI:10.1186/s12915-024-01972-4
Jijun Li, Yuting Zhang, Yahui Chen, Yijing Wang, Zhihua Zhou, Jinxing Tu, Liang Guo, Xuan Yao
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引用次数: 0

摘要

背景:甘蓝型油菜(Brassica napus L.,B. napus)在不同的栽培时期易受水涝胁迫的影响。因此,提高对水涝胁迫的抗性对实现油菜的高产稳产至关重要:结果:在这里,我们观察到两个油菜品种在涝胁迫下对根部反应的显著差异。与耐涝品种(桑塔纳)相比,敏感品种(23651)在涝胁迫下的细胞壁厚度和根系完整性下降更明显、更迅速。通过基于转录组数据的模块聚类分析,我们发现根部细胞壁多糖代谢对水涝胁迫有响应。研究发现,与耐涝品种相比,敏感品种的果胶含量明显降低。此外,转录组分析表明,在涝胁迫下,两个编码多聚半乳糖醛酸酶抑制蛋白 2(PGIP2)的同源基因在耐涝品种根部的表达高度上调,而这两个基因参与了多糖代谢途径。BnaPGIP2s可能通过抑制聚半乳糖醛酸酶(PGs)的活性,进而减少果胶骨架聚半乳糖醛酸的降解来赋予根系抗涝性:我们的研究结果表明,根中的细胞壁多糖在应对水涝胁迫中起着重要作用,为培育抗水涝的油菜品种提供了理论依据。
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The roles of cell wall polysaccharides in response to waterlogging stress in Brassica napus L. root.

Background: Brassica napus L. (B. napus) is susceptible to waterlogging stress during different cultivation periods. Therefore, it is crucial to enhance the resistance to waterlogging stress to achieve a high and stable yield of B. napus.

Results: Here we observed significant differences in the responses of two B. napus varieties in root under waterlogging stress. The sensitive variety (23651) exhibited a more pronounced and rapid reduction in cell wall thickness and root integrity compared with the tolerant variety (Santana) under waterlogging stress. By module clustering analysis based on transcriptome data, we identified that cell wall polysaccharide metabolism responded to waterlogging stress in root. It was found that pectin content was significantly reduced in the sensitive variety compared with the tolerant variety. Furthermore, transcriptome analysis revealed that the expression of two homologous genes encoding polygalacturonase-inhibiting protein 2 (PGIP2), involved in polysaccharide metabolic pathways, was highly upregulated in root of the tolerant variety under waterlogging stress. BnaPGIP2s probably confer waterlogging resistance by inhibiting the activity of polygalacturonases (PGs), which in turn reduces the degradation of the pectin backbone polygalacturonic acid.

Conclusions: Our findings demonstrate that cell wall polysaccharides in root plays a vital role in response to the waterlogging stress and provide a theoretical foundation for breeding waterlogging resistance in B. napus varieties.

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来源期刊
BMC Biology
BMC Biology 生物-生物学
CiteScore
7.80
自引率
1.90%
发文量
260
审稿时长
3 months
期刊介绍: BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.
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