氯霉素功能化介孔二氧化硅纳米粒子的合成、表征和细胞毒性评估

IF 4.9 3区 医学 Q1 PHARMACOLOGY & PHARMACY Pharmaceutics Pub Date : 2024-08-19 DOI:10.3390/pharmaceutics16081086
Juliana Camila Fischer Karnopp, Juliana Jorge, Jaqueline Rodrigues da Silva, Diego Boldo, Kristiane Fanti Del Pino Santos, Adriana Pereira Duarte, Gustavo Rocha de Castro, Ricardo Bentes de Azevedo, Ariadna Lafourcade Prada, Jesús Rafael Rodríguez Amado, Marco Antonio Utrera Martines
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引用次数: 0

摘要

本研究描述了氯丁氨嘧啶(CLB)功能化介孔二氧化硅纳米粒子(MSNs)的合成和表征,这种纳米粒子有望应用于癌症治疗。纳米颗粒的直径设计在 20 纳米到 50 纳米之间,以优化细胞吸收并避免从血液中快速清除。合成方法包括修改以前报道过的一种技术,以减小颗粒尺寸。通过各种技术,包括傅立叶变换红外光谱(FTIR)和元素分析,证实了 CLB 功能化的成功。评估了 CLB 功能化纳米粒子(MSN@NH2-CLB)对人肺腺癌细胞(A549)和结肠癌细胞(CT26WT)的细胞毒性。结果表明,与未结合的 CLB 相比,MSN@NH2-CLB 的细胞毒性明显更高,对癌细胞的选择性也高于正常细胞。这表明,MSN@NH2-CLB有望成为癌症靶向治疗的药物输送系统。
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Synthesis, Characterization, and Cytotoxicity Evaluation of Chlorambucil-Functionalized Mesoporous Silica Nanoparticles.

This study describes the synthesis and characterization of chlorambucil (CLB)-functionalized mesoporous silica nanoparticles (MSNs) for potential application in cancer therapy. The nanoparticles were designed with a diameter between 20 and 50 nm to optimize cellular uptake and avoid rapid clearance from the bloodstream. The synthesis method involved modifying a previously reported technique to reduce particle size. Successful functionalization with CLB was confirmed through various techniques, including Fourier transform infrared spectroscopy (FTIR) and elemental analysis. The cytotoxicity of the CLB-functionalized nanoparticles (MSN@NH2-CLB) was evaluated against human lung adenocarcinoma cells (A549) and colon carcinoma cells (CT26WT). The results suggest significantly higher cytotoxicity of MSN@NH2-CLB compared to unbound CLB, with improved selectivity towards cancer cells over normal cells. This suggests that MSN@NH2-CLB holds promise as a drug delivery system for targeted cancer therapy.

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来源期刊
Pharmaceutics
Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍: Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications,  and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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