Nicolas M S Galvez, Maegan L Sheehan, Allen Z Lin, Yi Cao, Evan C Lam, Abigail M Jackson, Alejandro B Balazs
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引用次数: 0
摘要
量化病毒增长率是了解进化动态和突变体逃避抗病毒药物的可能性的关键。确定进化逃逸路径及其对病毒适应性的影响,有助于开发抗病毒药物。就艾滋病毒而言,联合抗逆转录病毒疗法可以成功预防或治疗感染,但它依赖于严格的依从性来防止逃逸。在这里,我们提出了一种称为 QuickFit 的方法,通过大量平行的病毒培养物来精确测量生长率,从而量化病毒的适应性。QuickFit 始终如一地再现了通过传统方法获得的 HIV 生长测量结果,但通量明显更高,错误率更低。这种方法是快速、一致地评估病毒适应性的一种很有前途的工具。
QuickFit: A High-Throughput RT-qPCR-Based Assay to Quantify Viral Growth and Fitness In Vitro.
Quantifying viral growth rates is key to understanding evolutionary dynamics and the potential for mutants to escape antiviral drugs. Defining evolutionary escape paths and their impact on viral fitness allows for the development of drugs that are resistant to escape. In the case of HIV, combination antiretroviral therapy can successfully prevent or treat infection, but it relies on strict adherence to prevent escape. Here, we present a method termed QuickFit that enables the quantification of viral fitness by employing large numbers of parallel viral cultures to measure growth rates accurately. QuickFit consistently recapitulated HIV growth measurements obtained by traditional approaches, but with significantly higher throughput and lower rates of error. This method represents a promising tool for rapid and consistent evaluation of viral fitness.
期刊介绍:
Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.