Deep-learning optical flow for measuring velocity fields from experimental data†‡

Deep learning-based optical flow (DLOF) extracts features in adjacent video frames with deep convolutional neural networks. It uses those features to estimate the inter-frame motions of objects. We evaluate the ability of optical flow to quantify the spontaneous flows of microtubule (MT)-based active nematics under different labeling conditions, and compare its performance to particle image velocimetry (PIV). We obtain flow velocity ground truths either by performing semi-automated particle tracking on samples with sparsely labeled filaments, or from passive tracer beads. DLOF produces more accurate velocity fields than PIV for densely labeled samples. PIV cannot reliably distinguish contrast variations at high densities, particularly along the nematic director. DLOF overcomes this limitation. For sparsely labeled samples, DLOF and PIV produce comparable results, but DLOF gives higher-resolution fields. Our work establishes DLOF as a versatile tool for measuring fluid flows in a broad class of active, soft, and biophysical systems.