果蝇神经元的光遗传学控制:面向本科生和推广人员的实验室实践。

IF 1.6 Q2 EDUCATION, SCIENTIFIC DISCIPLINES Journal of Microbiology & Biology Education Pub Date : 2024-09-03 DOI:10.1128/jmbe.00086-24
Andrew D Renault
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引用次数: 0

摘要

在大班本科生中讲授神经科学方面的知识可能很困难,因为涉及到显微镜和电生理设备等设备的成本、掌握解剖或细胞内记录等技术所需的时间,以及使用脊椎动物时的伦理问题。在这里,我将介绍一种在果蝇身上使用行为读数和光遗传学的实践方法,这种方法可以以最低的成本实施,并减少了伦理方面的顾虑,而且主要使用的是观察技术。该实践可用于教授遗传学方面的知识以及操纵神经元活动以确定神经元功能的工具。该实践活动可根据不同的本科生水平和学习目标进行定制。
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Optogenetic control of Drosophila neurons: a laboratory practical for undergraduates and outreach.

Teaching aspects of neuroscience to large undergraduate classes can be difficult in terms of the cost of equipment involved such as microscopes and electrophysiology equipment, the time taken to master techniques such as dissection or intracellular recording, and ethical concerns when using vertebrates. Here, I describe a practical that uses behavioral readouts and optogenetics on Drosophila that can be implemented with minimal cost as well as reduced ethical concerns and uses mostly observational techniques. The practical can be used to teach aspects of genetics and the tools for manipulating neuronal activity for ascribing neuronal function. The practical can be customized to fit different undergraduate levels and learning objectives.

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来源期刊
Journal of Microbiology & Biology Education
Journal of Microbiology & Biology Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
3.00
自引率
26.30%
发文量
95
审稿时长
22 weeks
期刊最新文献
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