人类和大鼠的血脑屏障:三维成像透视

IF 3.3 Q2 ENGINEERING, BIOMEDICAL International Journal of Biomedical Imaging Pub Date : 2024-08-26 eCollection Date: 2024-01-01 DOI:10.1155/2024/4482931
Aiwen Chen, Gavin Volpato, Alice Pong, Emma Schofield, Jun Huang, Zizhao Qiu, George Paxinos, Huazheng Liang
{"title":"人类和大鼠的血脑屏障:三维成像透视","authors":"Aiwen Chen, Gavin Volpato, Alice Pong, Emma Schofield, Jun Huang, Zizhao Qiu, George Paxinos, Huazheng Liang","doi":"10.1155/2024/4482931","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> The blood-brain barrier (BBB) is part of the neurovascular unit (NVU) which plays a key role in maintaining homeostasis. However, its 3D structure is hardly known. The present study is aimed at imaging the BBB using tissue clearing and 3D imaging techniques in both human brain tissue and rat brain tissue. <b>Methods:</b> Both human and rat brain tissue were cleared using the CUBIC technique and imaged with either a confocal or two-photon microscope. Image stacks were reconstructed using Imaris. <b>Results:</b> Double staining with various antibodies targeting endothelial cells, basal membrane, pericytes of blood vessels, microglial cells, and the spatial relationship between astrocytes and blood vessels showed that endothelial cells do not evenly express CD31 and Glut1 transporter in the human brain. Astrocytes covered only a small portion of the vessels as shown by the overlap between GFAP-positive astrocytes and Collagen IV/CD31-positive endothelial cells as well as between GFAP-positive astrocytes and CD146-positive pericytes, leaving a big gap between their end feet. A similar structure was observed in the rat brain. <b>Conclusions:</b> The present study demonstrated the 3D structure of both the human and rat BBB, which is discrepant from the 2D one. Tissue clearing and 3D imaging are promising techniques to answer more questions about the real structure of biological specimens.</p>","PeriodicalId":47063,"journal":{"name":"International Journal of Biomedical Imaging","volume":"2024 ","pages":"4482931"},"PeriodicalIF":3.3000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368551/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Blood-Brain Barrier in Both Humans and Rats: A Perspective From 3D Imaging.\",\"authors\":\"Aiwen Chen, Gavin Volpato, Alice Pong, Emma Schofield, Jun Huang, Zizhao Qiu, George Paxinos, Huazheng Liang\",\"doi\":\"10.1155/2024/4482931\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Background:</b> The blood-brain barrier (BBB) is part of the neurovascular unit (NVU) which plays a key role in maintaining homeostasis. However, its 3D structure is hardly known. The present study is aimed at imaging the BBB using tissue clearing and 3D imaging techniques in both human brain tissue and rat brain tissue. <b>Methods:</b> Both human and rat brain tissue were cleared using the CUBIC technique and imaged with either a confocal or two-photon microscope. Image stacks were reconstructed using Imaris. <b>Results:</b> Double staining with various antibodies targeting endothelial cells, basal membrane, pericytes of blood vessels, microglial cells, and the spatial relationship between astrocytes and blood vessels showed that endothelial cells do not evenly express CD31 and Glut1 transporter in the human brain. Astrocytes covered only a small portion of the vessels as shown by the overlap between GFAP-positive astrocytes and Collagen IV/CD31-positive endothelial cells as well as between GFAP-positive astrocytes and CD146-positive pericytes, leaving a big gap between their end feet. A similar structure was observed in the rat brain. <b>Conclusions:</b> The present study demonstrated the 3D structure of both the human and rat BBB, which is discrepant from the 2D one. Tissue clearing and 3D imaging are promising techniques to answer more questions about the real structure of biological specimens.</p>\",\"PeriodicalId\":47063,\"journal\":{\"name\":\"International Journal of Biomedical Imaging\",\"volume\":\"2024 \",\"pages\":\"4482931\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368551/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biomedical Imaging\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/4482931\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomedical Imaging","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/4482931","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

摘要

背景:血脑屏障(BBB)是神经血管单元(NVU)的一部分,在维持体内平衡方面发挥着关键作用。然而,人们对其三维结构知之甚少。本研究旨在利用组织清除和三维成像技术对人脑组织和大鼠脑组织中的血脑屏障进行成像。研究方法使用 CUBIC 技术清除人脑和大鼠脑组织,并使用共聚焦显微镜或双光子显微镜成像。使用 Imaris 重建图像堆栈。结果用针对内皮细胞、基底膜、血管周细胞、小胶质细胞以及星形胶质细胞和血管之间空间关系的各种抗体进行双重染色,结果显示人脑内皮细胞并不均匀表达 CD31 和 Glut1 转运体。从 GFAP 阳性星形胶质细胞与胶原 IV/CD31 阳性内皮细胞之间以及 GFAP 阳性星形胶质细胞与 CD146 阳性周细胞之间的重叠可以看出,星形胶质细胞只覆盖了血管的一小部分,在它们的端脚之间留下了很大的空隙。在大鼠大脑中也观察到了类似的结构。结论本研究展示了人类和大鼠 BBB 的三维结构,这与二维结构有所不同。组织清除和三维成像技术有望回答更多有关生物标本真实结构的问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The Blood-Brain Barrier in Both Humans and Rats: A Perspective From 3D Imaging.

Background: The blood-brain barrier (BBB) is part of the neurovascular unit (NVU) which plays a key role in maintaining homeostasis. However, its 3D structure is hardly known. The present study is aimed at imaging the BBB using tissue clearing and 3D imaging techniques in both human brain tissue and rat brain tissue. Methods: Both human and rat brain tissue were cleared using the CUBIC technique and imaged with either a confocal or two-photon microscope. Image stacks were reconstructed using Imaris. Results: Double staining with various antibodies targeting endothelial cells, basal membrane, pericytes of blood vessels, microglial cells, and the spatial relationship between astrocytes and blood vessels showed that endothelial cells do not evenly express CD31 and Glut1 transporter in the human brain. Astrocytes covered only a small portion of the vessels as shown by the overlap between GFAP-positive astrocytes and Collagen IV/CD31-positive endothelial cells as well as between GFAP-positive astrocytes and CD146-positive pericytes, leaving a big gap between their end feet. A similar structure was observed in the rat brain. Conclusions: The present study demonstrated the 3D structure of both the human and rat BBB, which is discrepant from the 2D one. Tissue clearing and 3D imaging are promising techniques to answer more questions about the real structure of biological specimens.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
12.00
自引率
0.00%
发文量
11
审稿时长
20 weeks
期刊介绍: The International Journal of Biomedical Imaging is managed by a board of editors comprising internationally renowned active researchers. The journal is freely accessible online and also offered for purchase in print format. It employs a web-based review system to ensure swift turnaround times while maintaining high standards. In addition to regular issues, special issues are organized by guest editors. The subject areas covered include (but are not limited to): Digital radiography and tomosynthesis X-ray computed tomography (CT) Magnetic resonance imaging (MRI) Single photon emission computed tomography (SPECT) Positron emission tomography (PET) Ultrasound imaging Diffuse optical tomography, coherence, fluorescence, bioluminescence tomography, impedance tomography Neutron imaging for biomedical applications Magnetic and optical spectroscopy, and optical biopsy Optical, electron, scanning tunneling/atomic force microscopy Small animal imaging Functional, cellular, and molecular imaging Imaging assays for screening and molecular analysis Microarray image analysis and bioinformatics Emerging biomedical imaging techniques Imaging modality fusion Biomedical imaging instrumentation Biomedical image processing, pattern recognition, and analysis Biomedical image visualization, compression, transmission, and storage Imaging and modeling related to systems biology and systems biomedicine Applied mathematics, applied physics, and chemistry related to biomedical imaging Grid-enabling technology for biomedical imaging and informatics
期刊最新文献
Noninvasive Assessment of Cardiopulmonary Hemodynamics Using Cardiovascular Magnetic Resonance Pulmonary Transit Time. Comparison of 3D Gradient-Echo Versus 2D Sequences for Assessing Shoulder Joint Image Quality in MRI. The Blood-Brain Barrier in Both Humans and Rats: A Perspective From 3D Imaging. Presegmenter Cascaded Framework for Mammogram Mass Segmentation. An End-to-End CRSwNP Prediction with Multichannel ResNet on Computed Tomography.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1