Tania Quintana Hernandez, Asha Grover, Jamin Liu, Kenian Liu
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引用次数: 0
摘要
目的：荧光原位杂交（FISH）是检测 13q14 染色体缺失的一种可靠方法。目前，临床细胞遗传学实验室有三种用于 FISH 检测的商业探针：RB1、D13S319 和 D13S25，其中 D13S319 探针最常用于 13q 缺失的检测。在本研究中，我们比较了这三种探针在 13q 缺失阳性的 CLL 病例中的 FISH 检测结果。我们发现，D13S25探针组对双倍13q缺失百分比的检出率最高。
Comparison of Three FISH Probes Used By FISH Test For 13q Deletion in Chronic Lymphoid Leukemia.
Objectives: Fluorescence in situ hybridization (FISH) is a reliable method to detect a deletion on chromosome 13q14. Currently, three commercial probes are available for FISH testing in clinical cytogenetics laboratories, RB1, D13S319, and D13S25, with the D13S319 probe most commonly used for 13q deletion. In this study, we compared FISH test results among these three probes in CLL cases with positive 13q deletion. We found that the D13S25 probe set has the highest detection rate of the percentage of the biallelic 13q deletion.
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