Xiaomiao Cui, Zhiliang Gao, Kaijie Zhao, Ning Wang, Qun Yu, Muthupandian Ashokkumar, Jingcheng Hao, Jiwei Cui
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Sono-Assembly of Polyphenol–Protein Capsules for Enhanced Biocatalytic Cascades
We report a versatile approach for engineering protein capsules by harnessing the ultrasonication-mediated assembly (sono-assembly) of protein-stabilized emulsions for cascade biocatalysis. The capsules are assembled by coating volatile perfluorohexane emulsion with proteins aided by ultrasonication, followed by cross-linking proteins with polyphenols (e.g., tannic acid) and subsequent evaporation of the oil phase. When multiple enzymes are used as stabilizers for emulsion preparation, the resulting capsules can be utilized for biocatalytic cascades. The protein capsules as interfacial biocatalytic platforms can increase the interfacial area and maintain enzyme activity. Compared to capsules composed of a single type of enzymes, those incorporated with multiple enzymes demonstrate faster and more sensitive visual detection of substances (e.g., glucose). Moreover, the introduction of polyphenols not only enhances the stability of capsules but also participates in enzyme cascade reactions through the coordination of metal ions, generating reactive oxygen species (e.g., •OH) in tumor cells, potentiallly useful for chemodynamic therapy. The facile and scalable nature of the sono-assembly process, combined with the biocatalytic cascade properties, make these capsules promising candidates as microreactors for advanced biological and biomedical applications.
期刊介绍:
The journal Chemistry of Materials focuses on publishing original research at the intersection of materials science and chemistry. The studies published in the journal involve chemistry as a prominent component and explore topics such as the design, synthesis, characterization, processing, understanding, and application of functional or potentially functional materials. The journal covers various areas of interest, including inorganic and organic solid-state chemistry, nanomaterials, biomaterials, thin films and polymers, and composite/hybrid materials. The journal particularly seeks papers that highlight the creation or development of innovative materials with novel optical, electrical, magnetic, catalytic, or mechanical properties. It is essential that manuscripts on these topics have a primary focus on the chemistry of materials and represent a significant advancement compared to prior research. Before external reviews are sought, submitted manuscripts undergo a review process by a minimum of two editors to ensure their appropriateness for the journal and the presence of sufficient evidence of a significant advance that will be of broad interest to the materials chemistry community.