{"title":"淀粉分子量对淀粉卵磷脂复合纳米颗粒结肠靶向输送和促进 GLP-1 分泌的影响","authors":"","doi":"10.1016/j.foodhyd.2024.110589","DOIUrl":null,"url":null,"abstract":"<div><p><em>β</em>-lactoglobulin (<em>β</em>-LG) could stimulate enteroendocrine L cells which are located in the colon to secrete glucagon-like peptide-1 (GLP-1) to maintain glycemic homeostasis. In order to ensure that <em>β</em>-LG can intactly arrive in the colon through oral administration, colon-targeting delivery systems should be engineered. In this study, <em>β</em>-LG encapsulated nanoparticles were fabricated through molecular interaction and self-assembly using octenyl succinic anhydride (OSA) modified potato starch-lecithin complex (OPC) with different starch molecular weight (<em>Mw</em>). OPC with lower starch <em>Mw</em> exhibited stronger interaction with <em>β</em>-LG and its correspondent nanoparticles showed smaller diameter and higher structural compactness. Obtained OPC based nanoparticles were spherical, of Z-average diameter from 139.9 nm to 246.8 nm, of zeta-potential from −1.94 mV to −9.42 mV and of α value from 1.41 to 2.14. Additionally, OPC based nanoparticles with lower starch <em>Mw</em> exhibited excellent capacity for maintaining structure integrality in simulated upper gastrointestinal tract (GIT) environment. Optimized OPC based nanoparticles with 224.4 nm of diameter, −9.00 mV of zeta-potential and 2.14 of α value had well mucus-penetrating capacity (59.25%) and colon-targeting capacity (49.18% released in simulated colonic fluid). Moreover, the OPC based nanoparticles passed through the upper GIT signally stimulated GLP-1 secretion (improved 119.23%) primarily through <em>β</em>-LG targeting released in colon in the prophase and short-chain fatty acids and reducing sugar produced by colonic microbial degradation of OPC in the later phase. Altogether, <span>OPC</span> based nanoparticles have great potential of mucus penetrating and colon-targeting delivery systems for use in stimulating GLP-1 secretion which can support applications for maintaining glycemic homeostasis.</p></div>","PeriodicalId":320,"journal":{"name":"Food Hydrocolloids","volume":null,"pages":null},"PeriodicalIF":11.0000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of starch molecular weight on the colon-targeting delivery and promoting GLP-1 secretion of starch-lecithin complex nanoparticles\",\"authors\":\"\",\"doi\":\"10.1016/j.foodhyd.2024.110589\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>β</em>-lactoglobulin (<em>β</em>-LG) could stimulate enteroendocrine L cells which are located in the colon to secrete glucagon-like peptide-1 (GLP-1) to maintain glycemic homeostasis. In order to ensure that <em>β</em>-LG can intactly arrive in the colon through oral administration, colon-targeting delivery systems should be engineered. In this study, <em>β</em>-LG encapsulated nanoparticles were fabricated through molecular interaction and self-assembly using octenyl succinic anhydride (OSA) modified potato starch-lecithin complex (OPC) with different starch molecular weight (<em>Mw</em>). OPC with lower starch <em>Mw</em> exhibited stronger interaction with <em>β</em>-LG and its correspondent nanoparticles showed smaller diameter and higher structural compactness. Obtained OPC based nanoparticles were spherical, of Z-average diameter from 139.9 nm to 246.8 nm, of zeta-potential from −1.94 mV to −9.42 mV and of α value from 1.41 to 2.14. Additionally, OPC based nanoparticles with lower starch <em>Mw</em> exhibited excellent capacity for maintaining structure integrality in simulated upper gastrointestinal tract (GIT) environment. Optimized OPC based nanoparticles with 224.4 nm of diameter, −9.00 mV of zeta-potential and 2.14 of α value had well mucus-penetrating capacity (59.25%) and colon-targeting capacity (49.18% released in simulated colonic fluid). Moreover, the OPC based nanoparticles passed through the upper GIT signally stimulated GLP-1 secretion (improved 119.23%) primarily through <em>β</em>-LG targeting released in colon in the prophase and short-chain fatty acids and reducing sugar produced by colonic microbial degradation of OPC in the later phase. Altogether, <span>OPC</span> based nanoparticles have great potential of mucus penetrating and colon-targeting delivery systems for use in stimulating GLP-1 secretion which can support applications for maintaining glycemic homeostasis.</p></div>\",\"PeriodicalId\":320,\"journal\":{\"name\":\"Food Hydrocolloids\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":11.0000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Hydrocolloids\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0268005X24008634\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Hydrocolloids","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0268005X24008634","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Effect of starch molecular weight on the colon-targeting delivery and promoting GLP-1 secretion of starch-lecithin complex nanoparticles
β-lactoglobulin (β-LG) could stimulate enteroendocrine L cells which are located in the colon to secrete glucagon-like peptide-1 (GLP-1) to maintain glycemic homeostasis. In order to ensure that β-LG can intactly arrive in the colon through oral administration, colon-targeting delivery systems should be engineered. In this study, β-LG encapsulated nanoparticles were fabricated through molecular interaction and self-assembly using octenyl succinic anhydride (OSA) modified potato starch-lecithin complex (OPC) with different starch molecular weight (Mw). OPC with lower starch Mw exhibited stronger interaction with β-LG and its correspondent nanoparticles showed smaller diameter and higher structural compactness. Obtained OPC based nanoparticles were spherical, of Z-average diameter from 139.9 nm to 246.8 nm, of zeta-potential from −1.94 mV to −9.42 mV and of α value from 1.41 to 2.14. Additionally, OPC based nanoparticles with lower starch Mw exhibited excellent capacity for maintaining structure integrality in simulated upper gastrointestinal tract (GIT) environment. Optimized OPC based nanoparticles with 224.4 nm of diameter, −9.00 mV of zeta-potential and 2.14 of α value had well mucus-penetrating capacity (59.25%) and colon-targeting capacity (49.18% released in simulated colonic fluid). Moreover, the OPC based nanoparticles passed through the upper GIT signally stimulated GLP-1 secretion (improved 119.23%) primarily through β-LG targeting released in colon in the prophase and short-chain fatty acids and reducing sugar produced by colonic microbial degradation of OPC in the later phase. Altogether, OPC based nanoparticles have great potential of mucus penetrating and colon-targeting delivery systems for use in stimulating GLP-1 secretion which can support applications for maintaining glycemic homeostasis.
期刊介绍:
Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication.
The main areas of interest are:
-Chemical and physicochemical characterisation
Thermal properties including glass transitions and conformational changes-
Rheological properties including viscosity, viscoelastic properties and gelation behaviour-
The influence on organoleptic properties-
Interfacial properties including stabilisation of dispersions, emulsions and foams-
Film forming properties with application to edible films and active packaging-
Encapsulation and controlled release of active compounds-
The influence on health including their role as dietary fibre-
Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes-
New hydrocolloids and hydrocolloid sources of commercial potential.
The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.