一种产自海鲂幼虫的酸性异聚糖的结构特征和免疫增强特性

IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY Future Foods Pub Date : 2024-08-30 DOI:10.1016/j.fufo.2024.100439
Ju-Hwi Park , Jong-Jin Park , Jongbeom Chae , Seong Do Lee , Subin Gwon , Hunseong Kim , Ju Hyun Min , Young Hoon Jung , Joon Ha Lee , Wonyoung Lee , Jae Sam Hwang , Ibukunoluwa Fola Olawuyi , Ju-Ock Nam , Dongyup Hahn
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引用次数: 0

摘要

食用昆虫是具有多种健康益处的生物活性化合物的重要来源。本研究调查了从Protaetia brevitarsis seulensis幼虫(PBSL)中分离出的多糖的免疫调节特性。使用分子量截止膜对多糖进行了分馏,其中分子量较高的部分(H-PBSL,666.84 kDa)具有显著的免疫调节活性。甲基化和核磁共振结构分析表明,H-PBSL 是一种酸性异聚糖,结构复杂,由重复(1→4)连接的α-葡聚糖单位骨架组成,在 O-6 位高度支化,含有 T-α-Glcp、T-α-GlcpA 和 T-β-Galp。使用 RAW264.7 巨噬细胞评估了 H-PBSL 的免疫调节作用。H-PBSL 处理增强了吞噬活性,并增加了免疫调节剂和巨噬细胞活化表面分子的表达。此外,H-PBSL 还激活了 NF-κB 信号通路,表现为 IκB 和 NF-κB 磷酸化增加,NF-κB 从细胞膜转位到细胞核。此外,抑制 Toll 样受体 4(TLR4)可减少 H-PBSL 诱导的一氧化氮合成,这表明 TLR4 在 H-PBSL 介导的巨噬细胞活化中起着关键作用。这些发现凸显了 H-PBSL 作为一种从可食用昆虫中提取的新型免疫调节剂的潜力,并有可能应用于功能食品和营养保健品中。
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Structural characterization and immune-enhancing properties of an acidic heteroglycan from Protaetia brevitarsis seulensis larvae

Edible insects are a promising source of bioactive compounds with diverse health benefits. This study investigated the immunomodulatory properties of polysaccharides isolated from Protaetia brevitarsis seulensis larvae (PBSL). Polysaccharides were fractionated using a molecular weight cutoff membrane, and among these fractions, the higher molecular weight fraction (H-PBSL, 666.84 kDa) demonstrated significant immunomodulatory activity. Methylation and NMR structural analyses revealed H-PBSL to be an acidic heteroglycan with a complex structure, consisting of a backbone of repeating (1→4)-linked α-glucan units highly branched at O-6 positions with T-α-Glcp, T-α-GlcpA, and T-β-Galp. The immunomodulatory effects of H-PBSL were evaluated using RAW264.7 macrophage cells. H-PBSL treatment enhanced phagocytic activity and increased the expression of immune modulators and macrophage activation surface molecules. In addition, H-PBSL activated the NF-κB signaling pathway, evidenced by increased phosphorylation of IκB and NF-κB, and translocation of NF-κB from cytosol to nucleus. Moreover, inhibition of Toll-like receptor 4 (TLR4) reduced H-PBSL-induced nitric oxide synthesis, indicating TLR4′s pivotal role in H-PBSL-mediated macrophage activation. These findings highlight the potential of H-PBSL as a novel immunomodulatory agent derived from an edible insect, with possible applications in functional foods and nutraceuticals.

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来源期刊
Future Foods
Future Foods Agricultural and Biological Sciences-Food Science
CiteScore
8.60
自引率
0.00%
发文量
97
审稿时长
15 weeks
期刊介绍: Future Foods is a specialized journal that is dedicated to tackling the challenges posed by climate change and the need for sustainability in the realm of food production. The journal recognizes the imperative to transform current food manufacturing and consumption practices to meet the dietary needs of a burgeoning global population while simultaneously curbing environmental degradation. The mission of Future Foods is to disseminate research that aligns with the goal of fostering the development of innovative technologies and alternative food sources to establish more sustainable food systems. The journal is committed to publishing high-quality, peer-reviewed articles that contribute to the advancement of sustainable food practices. Abstracting and indexing: Scopus Directory of Open Access Journals (DOAJ) Emerging Sources Citation Index (ESCI) SCImago Journal Rank (SJR) SNIP
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