通过 DNA 酶纳米机器中的协同多催化核心提高核酸检测灵敏度

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY ChemBioChem Pub Date : 2024-09-05 DOI:10.1002/cbic.202400572

Zain Hussein, Lidia A. Golovina, Meera Alaji, Moustapha A. Y. Nour, Dmitry M. Kolpashchikov, Andrey B. Komissarov, Ahmed A. El-Deeb

{"title":"通过 DNA 酶纳米机器中的协同多催化核心提高核酸检测灵敏度","authors":"Zain Hussein, Lidia A. Golovina, Meera Alaji, Moustapha A. Y. Nour, Dmitry M. Kolpashchikov, Andrey B. Komissarov, Ahmed A. El-Deeb","doi":"10.1002/cbic.202400572","DOIUrl":null,"url":null,"abstract":"<p>We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.</p>","PeriodicalId":140,"journal":{"name":"ChemBioChem","volume":"25 23","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhancing Sensitivity in Nucleic Acid Detection via Collaborative Multiple Catalytic Cores in DNAzyme Nanomachines\",\"authors\":\"Zain Hussein, Lidia A. Golovina, Meera Alaji, Moustapha A. Y. Nour, Dmitry M. Kolpashchikov, Andrey B. Komissarov, Ahmed A. El-Deeb\",\"doi\":\"10.1002/cbic.202400572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.</p>\",\"PeriodicalId\":140,\"journal\":{\"name\":\"ChemBioChem\",\"volume\":\"25 23\",\"pages\":\"\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ChemBioChem\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202400572\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ChemBioChem","FirstCategoryId":"99","ListUrlMain":"https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202400572","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

我们介绍了一种新型多核 DNA 纳米机器（MDNM），它利用四种二元 DNA 酶进行核酸检测，无需前置扩增步骤。与单核系统相比，这一创新显著降低了检测限（LOD），降幅超过 5 倍。这缩短了所需的测试时间，凸显了 MDNM 在推进核酸检测方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

摘要图片

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Enhancing Sensitivity in Nucleic Acid Detection via Collaborative Multiple Catalytic Cores in DNAzyme Nanomachines

We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

ChemBioChem 生物-生化与分子生物学

CiteScore

6.10

自引率

3.10%

发文量

407

审稿时长

1 months

期刊介绍： ChemBioChem (Impact Factor 2018: 2.641) publishes important breakthroughs across all areas at the interface of chemistry and biology, including the fields of chemical biology, bioorganic chemistry, bioinorganic chemistry, synthetic biology, biocatalysis, bionanotechnology, and biomaterials. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies, and supported by the Asian Chemical Editorial Society (ACES).