通过 DNA 酶纳米机器中的协同多催化核心提高核酸检测灵敏度

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-09-05 DOI:10.1002/cbic.202400572

Zain Hussein, Lidia A Golovina, Meera Alaji, Moustapha A Y Nour, Dmitry M Kolpashchikov, Andrey B Komissarov, Ahmed A El-Deeb

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引用次数: 0

摘要

我们介绍了一种新型多核 DNA 纳米机器（MDNM），它利用四种二元 DNA 酶进行核酸检测，无需前置扩增步骤。与单核系统相比，这一创新显著降低了检测限（LOD），降幅超过 5 倍。这缩短了所需的测试时间，凸显了 MDNM 在推进核酸检测方面的潜力。

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Enhancing Sensitivity in Nucleic Acid Detection via Collaborative Multiple Catalytic Cores in DNAzyme Nanomachines.

We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5-fold, as compared to single-core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464