{"title":"关注头颈部癌症患者的肿瘤及其微环境免疫成员","authors":"","doi":"10.1016/j.prp.2024.155575","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Immune-related gene expression levels in the tumor microenvironment (TM) of head and neck squamous cell carcinoma (HNSCC) patients was compared.</p></div><div><h3>Materials and methods</h3><p>The <em>CD163</em>, <em>CD274</em>, <em>CD86</em>, <em>FUT4</em>, <em>FOXP3</em>, and <em>ITGAX</em> levels of HNSCC patients in their tumor tissues (n =76) and surrounding tissues adjacent to the tumor (n =76) were determined using quantitative real-time PCR (qRT-PCR). Changes in these genes were also evaluated by associating with demographical data of the patients.</p></div><div><h3>Results</h3><p><em>CD163</em>, <em>CD274</em>, <em>FUT4,</em> and <em>FOXP3</em> gene expression levels were significantly higher in tumor tissue than in surrounding tissue. <em>FUT4</em> fold change was statistically higher in patients with lymph node involvement. <em>CD86</em> expression was statistically lower in smokers of 50 boxes per year or more. <em>CD163</em>, <em>CD274</em>, and <em>FUT4</em> expressions were increased in response to the presence of extranodal extension (ENE).</p></div><div><h3>Conclusions</h3><p>These preliminary results demonstrate the alterations in expression levels of immunologic markers are associated with the clinical presentations of HNSCC.</p></div><div><h3>Availability of data and materials</h3><p>The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Focusing on tumor and it’s microenvironmental immune members for head and neck cancer patients\",\"authors\":\"\",\"doi\":\"10.1016/j.prp.2024.155575\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Immune-related gene expression levels in the tumor microenvironment (TM) of head and neck squamous cell carcinoma (HNSCC) patients was compared.</p></div><div><h3>Materials and methods</h3><p>The <em>CD163</em>, <em>CD274</em>, <em>CD86</em>, <em>FUT4</em>, <em>FOXP3</em>, and <em>ITGAX</em> levels of HNSCC patients in their tumor tissues (n =76) and surrounding tissues adjacent to the tumor (n =76) were determined using quantitative real-time PCR (qRT-PCR). Changes in these genes were also evaluated by associating with demographical data of the patients.</p></div><div><h3>Results</h3><p><em>CD163</em>, <em>CD274</em>, <em>FUT4,</em> and <em>FOXP3</em> gene expression levels were significantly higher in tumor tissue than in surrounding tissue. <em>FUT4</em> fold change was statistically higher in patients with lymph node involvement. <em>CD86</em> expression was statistically lower in smokers of 50 boxes per year or more. <em>CD163</em>, <em>CD274</em>, and <em>FUT4</em> expressions were increased in response to the presence of extranodal extension (ENE).</p></div><div><h3>Conclusions</h3><p>These preliminary results demonstrate the alterations in expression levels of immunologic markers are associated with the clinical presentations of HNSCC.</p></div><div><h3>Availability of data and materials</h3><p>The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.</p></div>\",\"PeriodicalId\":19916,\"journal\":{\"name\":\"Pathology, research and practice\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathology, research and practice\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0344033824004862\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathology, research and practice","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0344033824004862","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PATHOLOGY","Score":null,"Total":0}
Focusing on tumor and it’s microenvironmental immune members for head and neck cancer patients
Objective
Immune-related gene expression levels in the tumor microenvironment (TM) of head and neck squamous cell carcinoma (HNSCC) patients was compared.
Materials and methods
The CD163, CD274, CD86, FUT4, FOXP3, and ITGAX levels of HNSCC patients in their tumor tissues (n =76) and surrounding tissues adjacent to the tumor (n =76) were determined using quantitative real-time PCR (qRT-PCR). Changes in these genes were also evaluated by associating with demographical data of the patients.
Results
CD163, CD274, FUT4, and FOXP3 gene expression levels were significantly higher in tumor tissue than in surrounding tissue. FUT4 fold change was statistically higher in patients with lymph node involvement. CD86 expression was statistically lower in smokers of 50 boxes per year or more. CD163, CD274, and FUT4 expressions were increased in response to the presence of extranodal extension (ENE).
Conclusions
These preliminary results demonstrate the alterations in expression levels of immunologic markers are associated with the clinical presentations of HNSCC.
Availability of data and materials
The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
期刊介绍:
Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.