Weiyang Zhang, Qingyuan Liu, Jindong Zhang, Delin Wang
{"title":"敲除 FBXO45 可通过 ERK/Cyclin D1/CDK4 通路抑制膀胱癌的进展","authors":"Weiyang Zhang, Qingyuan Liu, Jindong Zhang, Delin Wang","doi":"10.56434/j.arch.esp.urol.20247707.111","DOIUrl":null,"url":null,"abstract":"<p><strong>Backgrounds: </strong>F-box protein 45 (<i>FBXO45</i>) has been implicated in the progression of several diseases. Whether <i>FBXO45</i> is involved in the development of bladder cancer remains unclear. Thus, this study focused on the effect of <i>FBXO45</i> on the malignant progression of bladder cancer cells.</p><p><strong>Methods: </strong><i>FBXO45</i> small-interference fragment was transfected into RT4 and 5637 cells by liposome-mediated transfection, and the knockdown efficiency of <i>FBXO45</i> was verified by Western blot assay. The growth rate between <i>FBXO45</i> knockdown cell lines and control cell lines was compared by counting kit 8 and plate cloning experiments. The motility of bladder cancer cells was observed via the Transwell test and Wound healing test. The effects of <i>FBXO45</i> silencing on apoptosis and cell division were confirmed by flow cytometry. Western blot assay was performed to determine the function of <i>FBXO45</i> knockdown on key proteins of cell apoptosis and the ERK/Cyclin D1/CDK4 pathway.</p><p><strong>Results: </strong>After <i>FBXO45</i> knockdown, the proliferation of bladder cancer cells was blocked (<i>p</i> < 0.01), and the migration and invasion abilities were reduced (<i>p</i> < 0.01). <i>FBXO45</i> knockdown reduced the number of S-phase cells (RT4, <i>p</i> < 0.01; 5637, <i>p</i> < 0.05) and enhanced the apoptotic rate (<i>p</i> < 0.01). <i>FBXO45</i> knockdown decreased the levels of p-ERK1/2, CDK4 and Cyclin D1 (<i>p</i> < 0.01).</p><p><strong>Conclusions: </strong>This study revealed that <i>FBXO45</i> plays a carcinogenic role in bladder cancer via the ERK/Cyclin D1/CDK4 pathway, which provides a reference for the clinical treatment of patients with bladder cancer.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"77 7","pages":"796-804"},"PeriodicalIF":0.6000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"FBXO45 Knockdown Restrains the Progression of Bladder Cancer via the ERK/Cyclin D1/CDK4 Pathway.\",\"authors\":\"Weiyang Zhang, Qingyuan Liu, Jindong Zhang, Delin Wang\",\"doi\":\"10.56434/j.arch.esp.urol.20247707.111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Backgrounds: </strong>F-box protein 45 (<i>FBXO45</i>) has been implicated in the progression of several diseases. Whether <i>FBXO45</i> is involved in the development of bladder cancer remains unclear. Thus, this study focused on the effect of <i>FBXO45</i> on the malignant progression of bladder cancer cells.</p><p><strong>Methods: </strong><i>FBXO45</i> small-interference fragment was transfected into RT4 and 5637 cells by liposome-mediated transfection, and the knockdown efficiency of <i>FBXO45</i> was verified by Western blot assay. The growth rate between <i>FBXO45</i> knockdown cell lines and control cell lines was compared by counting kit 8 and plate cloning experiments. The motility of bladder cancer cells was observed via the Transwell test and Wound healing test. The effects of <i>FBXO45</i> silencing on apoptosis and cell division were confirmed by flow cytometry. Western blot assay was performed to determine the function of <i>FBXO45</i> knockdown on key proteins of cell apoptosis and the ERK/Cyclin D1/CDK4 pathway.</p><p><strong>Results: </strong>After <i>FBXO45</i> knockdown, the proliferation of bladder cancer cells was blocked (<i>p</i> < 0.01), and the migration and invasion abilities were reduced (<i>p</i> < 0.01). <i>FBXO45</i> knockdown reduced the number of S-phase cells (RT4, <i>p</i> < 0.01; 5637, <i>p</i> < 0.05) and enhanced the apoptotic rate (<i>p</i> < 0.01). <i>FBXO45</i> knockdown decreased the levels of p-ERK1/2, CDK4 and Cyclin D1 (<i>p</i> < 0.01).</p><p><strong>Conclusions: </strong>This study revealed that <i>FBXO45</i> plays a carcinogenic role in bladder cancer via the ERK/Cyclin D1/CDK4 pathway, which provides a reference for the clinical treatment of patients with bladder cancer.</p>\",\"PeriodicalId\":48852,\"journal\":{\"name\":\"Archivos Espanoles De Urologia\",\"volume\":\"77 7\",\"pages\":\"796-804\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archivos Espanoles De Urologia\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.56434/j.arch.esp.urol.20247707.111\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"UROLOGY & NEPHROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archivos Espanoles De Urologia","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.56434/j.arch.esp.urol.20247707.111","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
FBXO45 Knockdown Restrains the Progression of Bladder Cancer via the ERK/Cyclin D1/CDK4 Pathway.
Backgrounds: F-box protein 45 (FBXO45) has been implicated in the progression of several diseases. Whether FBXO45 is involved in the development of bladder cancer remains unclear. Thus, this study focused on the effect of FBXO45 on the malignant progression of bladder cancer cells.
Methods: FBXO45 small-interference fragment was transfected into RT4 and 5637 cells by liposome-mediated transfection, and the knockdown efficiency of FBXO45 was verified by Western blot assay. The growth rate between FBXO45 knockdown cell lines and control cell lines was compared by counting kit 8 and plate cloning experiments. The motility of bladder cancer cells was observed via the Transwell test and Wound healing test. The effects of FBXO45 silencing on apoptosis and cell division were confirmed by flow cytometry. Western blot assay was performed to determine the function of FBXO45 knockdown on key proteins of cell apoptosis and the ERK/Cyclin D1/CDK4 pathway.
Results: After FBXO45 knockdown, the proliferation of bladder cancer cells was blocked (p < 0.01), and the migration and invasion abilities were reduced (p < 0.01). FBXO45 knockdown reduced the number of S-phase cells (RT4, p < 0.01; 5637, p < 0.05) and enhanced the apoptotic rate (p < 0.01). FBXO45 knockdown decreased the levels of p-ERK1/2, CDK4 and Cyclin D1 (p < 0.01).
Conclusions: This study revealed that FBXO45 plays a carcinogenic role in bladder cancer via the ERK/Cyclin D1/CDK4 pathway, which provides a reference for the clinical treatment of patients with bladder cancer.
期刊介绍：
Archivos Españoles de Urología published since 1944, is an international peer review, susbscription Journal on Urology with original and review articles on different subjets in Urology: oncology, endourology, laparoscopic, andrology, lithiasis, pediatrics , urodynamics,... Case Report are also admitted.
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