USP19 通过稳定 PARK7 在弥漫大 B 细胞淋巴瘤中发挥肿瘤促进作用。

Yaqing Li, Xiyang Liu, Yulai Li, Jieting Wang, Mengqian Zhang, Weili Xue, Mingzhi Zhang
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摘要

弥漫大B细胞淋巴瘤(DLBCL)是非霍奇金淋巴瘤中最常见的亚型,预后较差。基因表达谱交互分析(GEPIA)数据库的数据显示,DLBCL 组织中几种泛素特异性蛋白酶(USP)表达失调(DLBCL vs. 非 DLBCL = 47 vs. 337),其中包括 USP19(log2fold change = 1.17,P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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USP19 exerts a tumor-promoting role in diffuse large B cell lymphoma through stabilizing PARK7

Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma and is associated with a poor prognosis. Data from the Gene Expression Profiling Interactive Analysis (GEPIA) database revealed dysregulated expression of several ubiquitin-specific proteases (USPs) in DLBCL tissues (DLBCL vs. non-DLBCL = 47 vs. 337), including USP19 (log2fold change = 1.17, P < 0.05). USP19 is closely linked to tumorigenesis, but its role in DLBCL progression remains largely unknown. Here, we investigated the role of USP19 in DLBCL development. Genetic manipulation of USP19 using adenovirus-based vectors was performed in two DLBCL cell lines, SUDHL4 and DB cells. The results showed that USP19 knockdown suppressed the proliferation, anchorage-independent growth and xenograft tumor formation of DLBCL cells and arrested the cell cycle at the G1 stage. In parallel, DLBCL cells overexpressing USP19 acquired a more malignant phenotype. Next, to explore USP19 interactors, we performed co-immunoprecipitation/liquid chromatography–mass spectrometry and identified potential interacting proteins. Among them, Parkinson disease protein 7 (PARK7), a member of the peptidase C56 family known to be involved in carcinogenesis, was further validated to bind with and be stabilized by USP19. Additionally, we found that USP19 induced PARK7 deubiquitylation in both DLBCL cell lines, and PARK7 acted as a downstream effector of USP19 in regulating the growth of DLBCL cells. Collectively, USP19 exerts a tumor-promoting role in DLBCL through interacting with and stabilizing PARK7.

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