Edwin J Baldelomar, Hanwen Zhang, Daniel Thorek, Jennifer R Charlton, Patrick D Walker, Leslie D Wilson, Kasey C Emoto, Veronica Clavijo Jordan, David E Reichert, Kooresh Shoghi, Kevin M Bennett
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引用次数: 0
摘要
在这项研究中，我们开发了重组人阳离子铁蛋白（rHCF）作为一种造影剂，利用正电子发射断层扫描（PET）检测肾小球。我们首先在大肠杆菌中表达重组人铁蛋白（rHF），然后用铜-64（64Cu）对其进行功能化和放射性标记，形成 64Cu-rHCF。静脉注射的 64Cu-rHCF 与肾小球结合，并通过 PET 进行检测。在静脉注射 rHCF 后进行的亚慢性毒性研究显示没有明显毒性。rHCF的开发是将CF应用于临床检测人体肾小球数量的重要一步。
Development and Use of Human Recombinant 64Cu-rHCF as a Kidney Glomerulus-Targeted Contrast Agent for Positron Emission Tomography.
In this work, we develop recombinant human cationic ferritin (rHCF) as a contrast agent to detect glomeruli in the kidney using positron emission tomography (PET). We first expressed recombinant human ferritin (rHF) in E. coli and then functionalized and radiolabeled it with Copper-64 (64Cu) to form 64Cu-rHCF. Intravenously injected 64Cu-rHCF bound to kidney glomeruli and was detected by PET. A subchronic toxicity study after an intravenous injection of rHCF revealed no significant toxicity. The development of rHCF is an important step toward the potential clinical translation of CF to detect the nephron number in humans.
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