通过磁共振成像检测 MASLD 动物模型中肝脏脂质氧化的改变。

IF 11.7 1区 医学 Q1 CELL BIOLOGY Cell Reports Medicine Pub Date : 2024-09-17 Epub Date: 2024-09-05 DOI:10.1016/j.xcrm.2024.101714
Marc McLeod, Mario C Chang, Anna Rushin, Mukundan Ragavan, Rohit Mahar, Gaurav Sharma, Arshee Badar, Anthony Giacalone, Max E Glanz, Vinay R Malut, Dalton Graham, Nishanth E Sunny, James A Bankson, Kenneth Cusi, Matthew E Merritt
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引用次数: 0

摘要

代谢功能障碍相关性脂肪性肝病(MASLD)的发病率逐年上升,影响着超过三分之一的美国成年人。代谢性脂肪肝可发展为代谢功能障碍相关性脂肪性肝炎(MASH),其特征是严重的肝细胞损伤、炎症和最终的晚期纤维化或肝硬化。预计到 2030 年,MASH 将成为肝移植的主要病因。虽然 MASLD/MASH 的病因尚不完全清楚,但脂肪酸氧化失调与疾病的发病机制有关。在此,我们开发了一种方法,通过[D15]辛酸盐代谢成氘化水,并用氘磁共振方法进行检测,来估算肝脏的β氧化作用。MASLD小鼠模型的肝脏灌注显示肝脏β氧化失调,这些发现与体内成像结果相吻合。高脂饮食诱导的MASLD小鼠研究表明,脂肪肝中β氧化效率的降低可作为MASLD进展的指标。此外,我们的方法为确定肝脏β氧化效率提供了一种可临床应用的成像方法。
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Detecting altered hepatic lipid oxidation by MRI in an animal model of MASLD.

Metabolic dysfunction-associated steatotic liver disease (MASLD) prevalence is increasing annually and affects over a third of US adults. MASLD can progress to metabolic dysfunction-associated steatohepatitis (MASH), characterized by severe hepatocyte injury, inflammation, and eventual advanced fibrosis or cirrhosis. MASH is predicted to become the primary cause of liver transplant by 2030. Although the etiology of MASLD/MASH is incompletely understood, dysregulated fatty acid oxidation is implicated in disease pathogenesis. Here, we develop a method for estimating hepatic β-oxidation from the metabolism of [D15]octanoate to deuterated water and detection with deuterium magnetic resonance methods. Perfused livers from a mouse model of MASLD reveal dysregulated hepatic β-oxidation, findings that corroborate in vivo imaging. The high-fat-diet-induced MASLD mouse studies indicate that decreased β-oxidative efficiency in the fatty liver could serve as an indicator of MASLD progression. Furthermore, our method provides a clinically translatable imaging approach for determining hepatic β-oxidation efficiency.

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来源期刊
Cell Reports Medicine
Cell Reports Medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
15.00
自引率
1.40%
发文量
231
审稿时长
40 days
期刊介绍: Cell Reports Medicine is an esteemed open-access journal by Cell Press that publishes groundbreaking research in translational and clinical biomedical sciences, influencing human health and medicine. Our journal ensures wide visibility and accessibility, reaching scientists and clinicians across various medical disciplines. We publish original research that spans from intriguing human biology concepts to all aspects of clinical work. We encourage submissions that introduce innovative ideas, forging new paths in clinical research and practice. We also welcome studies that provide vital information, enhancing our understanding of current standards of care in diagnosis, treatment, and prognosis. This encompasses translational studies, clinical trials (including long-term follow-ups), genomics, biomarker discovery, and technological advancements that contribute to diagnostics, treatment, and healthcare. Additionally, studies based on vertebrate model organisms are within the scope of the journal, as long as they directly relate to human health and disease.
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