Syed Rashel Kabir , Mohammad Taufiq Alam , Md Belal Uddin
{"title":"芦笋氯化银纳米粒子和山柰介导的银/氯化银纳米粒子对人类肝癌和肺癌细胞株的抑制作用","authors":"Syed Rashel Kabir , Mohammad Taufiq Alam , Md Belal Uddin","doi":"10.1016/j.bbrep.2024.101818","DOIUrl":null,"url":null,"abstract":"<div><p>Recently, we have reported that biogenic silver/silver chloride nanoparticles from <em>Asparagus racemosus</em> (<em>A. racemosus</em>-AgCl-NPs) and <em>Kaempferia rotunda</em> (<em>K. rotunda</em>-Ag/AgCl-NPs) inhibited different cancer cells by inducing apoptosis and several genes alteration. Here for the first time, we assessed the effects of these two nanoparticles on human lung (A549) and hepatocellular (SMMC-7721) carcinoma cell lines. <em>A. racemosus-</em>AgCl-NPs and <em>K. rotunda</em>-Ag/AgCl-NPs inhibited A549 cell growth with IC<sub>50</sub> values of 22.7 and 59.7 μg/ml and the calculated IC<sub>50</sub> values for SMMC-7721 cell were 89.3 and 126.3 μg/ml, respectively. <em>A. racemosus</em>-AgCl-NPs exerted higher cytotoxicity against HEK293T cells than doxorubicin and <em>K. rotunda</em>-Ag/AgCl-NPs. Both the nanoparticles induced apoptosis in A549 and SMMC-7721 cell lines. A significant rise of early apoptotic cells and late apoptotic cells was found for A549 cells after treatment with <em>A. racemosus</em>-AgCl-NPs and stained with FITC-annexin V/PI. Apoptosis in A549 cells was further confirmed by monitoring the alteration of the expression level of several genes using real-time PCR and cell cycle arrest by flowcytometry after treatment with <em>A. racemosus</em>-AgCl-NPs. The expression of STAT-3, TNFα, and EGFR genes was decreased with the increase of caspase-8, FAS, and FADD gene expression. G<sub>2</sub>/M cell cycle phase was arrested after treatment of A549 cells with <em>A. racemosus</em>-AgCl-NPs.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"40 ","pages":"Article 101818"},"PeriodicalIF":2.3000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001821/pdfft?md5=b85d7f30e299f375490840e9faab62f5&pid=1-s2.0-S2405580824001821-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Asparagus racemosus silver chloride nanoparticles and Kaempferia rotunda mediated silver/silver chloride nanoparticles inhibit human hepatocellular and lung cancer cell lines\",\"authors\":\"Syed Rashel Kabir , Mohammad Taufiq Alam , Md Belal Uddin\",\"doi\":\"10.1016/j.bbrep.2024.101818\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Recently, we have reported that biogenic silver/silver chloride nanoparticles from <em>Asparagus racemosus</em> (<em>A. racemosus</em>-AgCl-NPs) and <em>Kaempferia rotunda</em> (<em>K. rotunda</em>-Ag/AgCl-NPs) inhibited different cancer cells by inducing apoptosis and several genes alteration. Here for the first time, we assessed the effects of these two nanoparticles on human lung (A549) and hepatocellular (SMMC-7721) carcinoma cell lines. <em>A. racemosus-</em>AgCl-NPs and <em>K. rotunda</em>-Ag/AgCl-NPs inhibited A549 cell growth with IC<sub>50</sub> values of 22.7 and 59.7 μg/ml and the calculated IC<sub>50</sub> values for SMMC-7721 cell were 89.3 and 126.3 μg/ml, respectively. <em>A. racemosus</em>-AgCl-NPs exerted higher cytotoxicity against HEK293T cells than doxorubicin and <em>K. rotunda</em>-Ag/AgCl-NPs. Both the nanoparticles induced apoptosis in A549 and SMMC-7721 cell lines. A significant rise of early apoptotic cells and late apoptotic cells was found for A549 cells after treatment with <em>A. racemosus</em>-AgCl-NPs and stained with FITC-annexin V/PI. Apoptosis in A549 cells was further confirmed by monitoring the alteration of the expression level of several genes using real-time PCR and cell cycle arrest by flowcytometry after treatment with <em>A. racemosus</em>-AgCl-NPs. The expression of STAT-3, TNFα, and EGFR genes was decreased with the increase of caspase-8, FAS, and FADD gene expression. G<sub>2</sub>/M cell cycle phase was arrested after treatment of A549 cells with <em>A. racemosus</em>-AgCl-NPs.</p></div>\",\"PeriodicalId\":8771,\"journal\":{\"name\":\"Biochemistry and Biophysics Reports\",\"volume\":\"40 \",\"pages\":\"Article 101818\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-09-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2405580824001821/pdfft?md5=b85d7f30e299f375490840e9faab62f5&pid=1-s2.0-S2405580824001821-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry and Biophysics Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405580824001821\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580824001821","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Asparagus racemosus silver chloride nanoparticles and Kaempferia rotunda mediated silver/silver chloride nanoparticles inhibit human hepatocellular and lung cancer cell lines
Recently, we have reported that biogenic silver/silver chloride nanoparticles from Asparagus racemosus (A. racemosus-AgCl-NPs) and Kaempferia rotunda (K. rotunda-Ag/AgCl-NPs) inhibited different cancer cells by inducing apoptosis and several genes alteration. Here for the first time, we assessed the effects of these two nanoparticles on human lung (A549) and hepatocellular (SMMC-7721) carcinoma cell lines. A. racemosus-AgCl-NPs and K. rotunda-Ag/AgCl-NPs inhibited A549 cell growth with IC50 values of 22.7 and 59.7 μg/ml and the calculated IC50 values for SMMC-7721 cell were 89.3 and 126.3 μg/ml, respectively. A. racemosus-AgCl-NPs exerted higher cytotoxicity against HEK293T cells than doxorubicin and K. rotunda-Ag/AgCl-NPs. Both the nanoparticles induced apoptosis in A549 and SMMC-7721 cell lines. A significant rise of early apoptotic cells and late apoptotic cells was found for A549 cells after treatment with A. racemosus-AgCl-NPs and stained with FITC-annexin V/PI. Apoptosis in A549 cells was further confirmed by monitoring the alteration of the expression level of several genes using real-time PCR and cell cycle arrest by flowcytometry after treatment with A. racemosus-AgCl-NPs. The expression of STAT-3, TNFα, and EGFR genes was decreased with the increase of caspase-8, FAS, and FADD gene expression. G2/M cell cycle phase was arrested after treatment of A549 cells with A. racemosus-AgCl-NPs.
期刊介绍：
Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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