芦笋氯化银纳米粒子和山柰介导的银/氯化银纳米粒子对人类肝癌和肺癌细胞株的抑制作用

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-09-05 DOI:10.1016/j.bbrep.2024.101818
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引用次数: 0

摘要

最近,我们报道了来自天门冬(A. racemosus-AgCl-NPs)和山柰(K. rotunda-Ag/AgCl-NPs)的生物银/氯化银纳米粒子通过诱导细胞凋亡和多个基因的改变来抑制不同的癌细胞。在此,我们首次评估了这两种纳米粒子对人类肺癌(A549)和肝癌(SMMC-7721)细胞系的影响。A. racemosus-AgCl-NPs和K. rotunda-Ag/AgCl-NPs抑制A549细胞生长的IC50值分别为22.7和59.7 μg/ml,对SMMC-7721细胞的计算IC50值分别为89.3和126.3 μg/ml。A. racemosus-AgCl-NPs 对 HEK293T 细胞的细胞毒性高于多柔比星和 K. rotunda-Ag/AgCl-NPs。这两种纳米粒子都能诱导 A549 和 SMMC-7721 细胞系凋亡。在使用A. racemosus-AgCl-NPs处理A549细胞并用FITC-annexin V/PI染色后,发现早期凋亡细胞和晚期凋亡细胞明显增加。通过使用实时 PCR 检测多个基因表达水平的变化,以及使用流式细胞仪检测 A549 细胞处理后细胞周期的停滞,进一步证实了 A549 细胞的凋亡。STAT-3、TNFα和表皮生长因子受体(EGFR)基因的表达减少,而Caspase-8、FAS和FADD基因的表达增加。用 A. racemosus-AgCl-NPs 处理 A549 细胞后,G2/M 细胞周期阶段停止。
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Asparagus racemosus silver chloride nanoparticles and Kaempferia rotunda mediated silver/silver chloride nanoparticles inhibit human hepatocellular and lung cancer cell lines

Recently, we have reported that biogenic silver/silver chloride nanoparticles from Asparagus racemosus (A. racemosus-AgCl-NPs) and Kaempferia rotunda (K. rotunda-Ag/AgCl-NPs) inhibited different cancer cells by inducing apoptosis and several genes alteration. Here for the first time, we assessed the effects of these two nanoparticles on human lung (A549) and hepatocellular (SMMC-7721) carcinoma cell lines. A. racemosus-AgCl-NPs and K. rotunda-Ag/AgCl-NPs inhibited A549 cell growth with IC50 values of 22.7 and 59.7 μg/ml and the calculated IC50 values for SMMC-7721 cell were 89.3 and 126.3 μg/ml, respectively. A. racemosus-AgCl-NPs exerted higher cytotoxicity against HEK293T cells than doxorubicin and K. rotunda-Ag/AgCl-NPs. Both the nanoparticles induced apoptosis in A549 and SMMC-7721 cell lines. A significant rise of early apoptotic cells and late apoptotic cells was found for A549 cells after treatment with A. racemosus-AgCl-NPs and stained with FITC-annexin V/PI. Apoptosis in A549 cells was further confirmed by monitoring the alteration of the expression level of several genes using real-time PCR and cell cycle arrest by flowcytometry after treatment with A. racemosus-AgCl-NPs. The expression of STAT-3, TNFα, and EGFR genes was decreased with the increase of caspase-8, FAS, and FADD gene expression. G2/M cell cycle phase was arrested after treatment of A549 cells with A. racemosus-AgCl-NPs.

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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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