在深静脉血栓形成的小鼠模型中使用微流体产生的微气泡进行声波溶栓。

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL Annals of Biomedical Engineering Pub Date : 2024-09-09 DOI:10.1007/s10439-024-03609-7
Yanjun Xie, Yi Huang, Hugo C S Stevenson, Li Yin, Kaijie Zhang, Zain Husain Islam, William Aaron Marcum, Campbell Johnston, Nicholas Hoyt, Eric William Kent, Bowen Wang, John A Hossack
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引用次数: 0

摘要

鉴于抗凝剂和溶栓药物的相关风险,人们需要安全有效的方法来治疗深静脉血栓(DVT),这促使人们研究创新的方法来溶解血栓。为了应对这一挑战,人们正在探索一种将微气泡、超声波和溶栓药物结合在一起的技术,以促进血栓的积极溶解。先前的研究表明,在体外或动脉模型中,相对较大的微气泡可加速溶解过程。然而,在血流速度无法与之相比的静脉血栓栓塞疾病中,必须对使用大型微气泡的声波溶栓进行评估。在这项研究中,声波溶栓的疗效在已有深静脉血栓的小鼠模型中得到了验证。在治疗过程中,通过尾静脉导管给小鼠注射直径为 18 μm 的微流控气泡和重组组织纤溶酶原激活剂(rt-PA)30 分钟,同时对小鼠腹部区域进行超声波照射。治疗前后均进行了三维超声扫描,以进行量化。超声溶栓治疗后的动物血栓残余体积为20%,而未接受治疗时为52%(P = 0.012 0.05),表明深静脉血栓体积显著减少。组织切片的组织学分析证实了治疗后深静脉血栓体积的减少。因此,由微流体设备产生的大型微气泡有望用于超声辅助治疗,以解决与静脉血栓栓塞有关的问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Sonothrombolysis Using Microfluidically Produced Microbubbles in a Murine Model of Deep Vein Thrombosis.

The need for safe and effective methods to manage deep vein thrombosis (DVT), given the risks associated with anticoagulants and thrombolytic agents, motivated research into innovative approaches to resolve blood clots. In response to this challenge, sonothrombolysis is being explored as a technique that combines microbubbles, ultrasound, and thrombolytic agents to facilitate the aggressive dissolution of thrombi. Prior studies have indicated that relatively large microbubbles accelerate the dissolution process, either in an in vitro or an arterial model. However, sonothrombolysis using large microbubbles must be evaluated in venous thromboembolism diseases, where blood flow velocity is not comparable. In this study, the efficacy of sonothrombolysis was validated in a murine model of pre-existing DVT. During therapy, microfluidically produced microbubbles of 18 μm diameter and recombinant tissue plasminogen activator (rt-PA) were administered through a tail vein catheter for 30 min, while ultrasound was applied to the abdominal region of the mice. Three-dimensional ultrasound scans were performed before and after therapy for quantification. The residual volume of the thrombi was 20% in animals post sonothrombolysis versus 52% without therapy ( p = 0.012 < 0.05 ), indicating a significant reduction in DVT volume. Histological analysis of tissue sections confirmed a reduction in DVT volume post-therapy. Therefore, large microbubbles generated from a microfluidic device show promise in ultrasound-assisted therapy to address concerns related to venous thromboembolism.

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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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