鹅源性脂肪连接蛋白肽 gADP3 对 LPS 诱导的鹅肝炎症损伤的影响

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE British Poultry Science Pub Date : 2024-09-09 DOI:10.1080/00071668.2024.2393960
B Ma, Y Zheng, S Liu, Y Qiu, X Xing, M Gao, Z Cao, X Luan
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引用次数: 0

摘要

1.本研究评估了多肽 gADP3 对脂多糖(LPS)诱导的肝脏炎症损伤的影响和作用机制。 2. 通过腹腔注射 LPS 和 gADP3 诱导鹅肝脏炎症损伤,并使用脂肪直通素受体激动剂 AdipoRon(阳性对照)进行潜在的改善。测定了血清炎症因子水平、肝功能相关生化指标和肝组织中氧化应激相关生化指标。还检测了肝组织中脂联素及其受体、炎症和氧化应激相关基因以及参与脂联素、炎症和氧化应激信号通路的关键信号分子的表达水平。多肽 gADP3 可减轻炎症细胞浸润和肝脏炎症变化,逆转 LPS 诱导的血清白蛋白(ALB)、总蛋白(TP)、丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)含量或活性的下降,并提高抗氧化酶 CAT(过氧化氢酶)、SOD(超氧化物歧化酶)和 GSH-Px(谷胱甘肽过氧化物酶)的活性。多肽 gADP3 能上调肝组织中抗氧化酶相关基因 GCLC、HO-1 和 NQO1 的表达,降低炎症因子如 TNF-α、IL-1β、IL-6、IFN-γ 和 TGF-β 的水平,降低炎症相关基因 TNF-α、IL-1β、iNOS 和 TGF-β 的 mRNA 表达水平。此外,它还能提高脂肪粘连素及其受体的 mRNA 和蛋白表达水平,以及脂肪粘连素信号通路中的关键分子,如 AMPK 和 PPARα。此外,gADP3 还能逆转 LPS 处理引起的肝组织中炎症和氧化应激信号通路相关基因 P38MAPK、NF-κBP65、TLR4 和 Nrf2 的 mRNA 或蛋白表达变化。 总之,鹅源性脂肪连接蛋白肽 gADP3 与脂肪连接蛋白受体激动剂 AdipoRon 相似,都能减轻 LPS 引起的鹅肝脏炎症损伤。
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Effect of goose-derived adiponectin peptide gADP3 on LPS-induced inflammatory injury in goose liver.

1. This study evaluated the effects and mechanisms of action of the peptide gADP3 on hepatic inflammatory injury induced by lipopolysaccharide (LPS).2. Hepatic inflammatory injury was induced in geese by intraperitoneal injection of LPS and gADP3, and the adiponectin receptor agonist AdipoRon (positive control) was used for potential amelioration. Serum inflammatory factor levels, liver function-related biochemical indicators and oxidative stress-related biochemical parameters in the liver tissues were determined. The expression levels of adiponectin and its receptors, inflammation and oxidative stress-related genes and key signalling molecules involved in adiponectin, inflammation and oxidative stress signalling pathways in liver tissues were detected.3. The peptide gADP3 alleviated inflammatory cell infiltration and hepatic inflammatory changes, reversed the decrease in serum albumin (ALB), total protein (TP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) content or activity induced by LPS and increased the activity of the antioxidant enzymes CAT (catalase), SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase).4. The peptide gADP3 upregulated the expression of antioxidant enzyme-related genes GCLC, HO-1 and NQO1 in liver tissues, decreased the levels of inflammatory factors like TNF-α, IL-1β, IL-6, IFN-γ and TGF-β and reduced mRNA expression levels of inflammatory-related genes TNF-α, IL-1β, iNOS and TGF-β. Additionally, it increased the mRNA and protein expression levels of adiponectin and its receptors, as well as key molecules in the adiponectin signalling pathway like AMPK and PPARα. In addition, gADP3 reversed the changes in mRNA or protein expression of inflammatory and oxidative stress signalling pathway-related genes P38MAPK, NF-κBP65, TLR4 and Nrf2 in liver tissues caused by LPS treatment.5. In conclusion, goose-derived adiponectin peptide gADP3, similar to the adiponectin receptor agonist AdipoRon, attenuated LPS-induced hepatic inflammatory injury in geese.

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来源期刊
British Poultry Science
British Poultry Science 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
88
审稿时长
4.5 months
期刊介绍: From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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