慢性肾脏病中的基质la蛋白和一氧化氮合成酶-3基因变异及其与心血管风险的关系

IF 1.7 Q3 UROLOGY & NEPHROLOGY International Journal of Nephrology Pub Date : 2024-08-30 eCollection Date: 2024-01-01 DOI:10.1155/2024/3850055
G Priyadarshini, Sreejith Parameswaran, Jayaprakash Sahoo, Sandhiya Selvarajan, Ananthakrishnan Ramesh, Medha Rajappa
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引用次数: 0

摘要

背景:慢性肾脏病(CKD)是指肾实质逐渐恶化,肾小球功能衰退。慢性肾脏病患者发生心血管事件的风险大大增加。迄今为止,人们对 CKD 与心血管疾病(CVD)之间的复杂联系还不甚了解:我们旨在研究南印度泰米尔人基质格拉蛋白(MGP)基因 rs1800801、rs1800802 和 rs4236 以及一氧化氮合酶-3(NOS3)基因 rs1799983 和 rs2070744 的遗传变异对 CKD 风险及其相关心血管合并症的影响:本研究招募了 185 名 CKD 患者和 185 名对照组。用超声波测量了肱动脉的血流介导扩张(FMD)。用酶联免疫吸附法测定循环中 MGP 和一氧化氮(NO)的水平。通过实时 PCR 进行基因分型:结果:我们观察到 NOS3(rs2070744)的 TT 和 CT 基因型分布存在明显差异,表明患慢性肾脏病的风险增加。与对照组相比,CKD 病例的 NO 水平明显下降。我们还发现,MGP 多态性(1-rs4236;2-rs1800801;3-rs1800802)的 TTA 和 CCG 单倍型的分布在各组间存在明显差异,表明 CKD 风险增加。MGP的CT基因型(rs4236)和NOS3的CT基因型(rs2070744)变异与FMD下降有关,表明内皮功能障碍是心血管疾病的先兆:我们得出的结论是,MGP 和 NOS3 基因变异会增加南印度泰米尔人患慢性肾脏病及其相关心血管并发症的风险。
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Matrix Gla Protein and Nitric Oxide Synthase-3 Genetic Variants in Chronic Kidney Disease and Their Relation with Cardiovascular Risk.

Background: Chronic kidney disease (CKD) is defined by gradual deterioration of renal parenchyma and decline of functioning nephrons. The risk of cardiovascular events is drastically increased in patients with CKD. This complicated link of CKD and cardiovascular disease (CVD) is not well understood till date.

Objective: We aim to study the influence of genetic variants of matrix Gla protein (MGP) gene rs1800801, rs1800802, and rs4236 and nitric oxide synthase-3 (NOS3) gene rs1799983 and rs2070744 on the risk of CKD and its associated cardiovascular comorbidity in South Indian Tamils.

Methods: One hundred and eighty-five CKD patients and 185 controls were recruited in this research. Flow-mediated dilatation (FMD) of brachial artery was measured ultrasonically. Circulating levels of MGP and nitric oxide (NO) were measured by ELISA. Genotyping was done by real-time PCR.

Results: We observed a significant difference in the distribution of TT and CT genotypes of NOS3 (rs2070744), indicating an increase in the risk of CKD. NO level was significantly decreased in CKD cases than controls. We also found a significant difference in the distribution of TTA and CCG haplotypes of MGP polymorphisms (1-rs4236; 2-rs1800801; 3-rs1800802) between the groups, indicating an increase in the risk of CKD. CT genotype of MGP (rs4236) and CT genotype of NOS3 (rs2070744) variants were found to be associated with decreased FMD, indicating endothelial dysfunction, the harbinger of CVD.

Conclusion: We conclude that genetic variants of MGP and NOS3 enhance the risk of CKD and its associated cardiovascular comorbidity in South Indian Tamils.

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来源期刊
International Journal of Nephrology
International Journal of Nephrology UROLOGY & NEPHROLOGY-
CiteScore
3.40
自引率
4.80%
发文量
44
审稿时长
17 weeks
期刊介绍: International Journal of Nephrology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies focusing on the prevention, diagnosis, and management of kidney diseases and associated disorders. The journal welcomes submissions related to cell biology, developmental biology, genetics, immunology, pathology, pathophysiology of renal disease and progression, clinical nephrology, dialysis, and transplantation.
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