{"title":"用远紫外线、紫外线和紫外线辐照 C. auris、S. cerevisiae 和 C. cladosporioides 的敏感性分析。","authors":"Anna-Maria Gierke, Martin Hessling","doi":"10.20411/pai.v9i2.723","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The World Health Organization has published a list of pathogenic fungi with prior-itizing groups and calls for research and development of antifungal measures, with <i>Candida auris</i> belonging to the group with high priority.</p><p><strong>Methods: </strong>The photosensitivity towards short wavelength ultraviolet irradiation (Far-UVC, UVC, and UVB) was investigated and compared to other yeasts (<i>Saccharomyces cerevisiae</i>) and a mold (<i>Cladosporium cladosporioides</i>). The observed 1-log reduction doses were compared to literature values of other representatives of the genus <i>Candida</i>, but also with <i>S. cerevisiae, Aspergillus niger,</i> and <i>A. fumigatus</i>.</p><p><strong>Results: </strong>For the determined 1-log reduction doses, an increase with higher wavelengths was observed. A 1-log reduction dose of 4.3 mJ/cm<sup>2</sup> was determined for <i>C. auris</i> when irradiated at 222 nm, a dose of 6.1 mJ/cm<sup>2</sup> at 254 nm and a 1-log reduction dose of 51.3 mJ/cm<sup>2</sup> was required when irradiated with UVB.</p><p><strong>Conclusions: </strong>It was observed that <i>S. cerevisiae</i> is a possible surrogate for <i>C. auris</i> for irradiation with Far-UVC and UVB due to close 1-log reduction doses. No surrogate suitability was verified for <i>C. cladosporioides</i> in relation to <i>A. niger</i> and <i>A. fumigatus</i> for irradiation with a wavelength of 254 nm and for <i>A. niger</i> at 222 nm.</p>","PeriodicalId":36419,"journal":{"name":"Pathogens and Immunity","volume":"9 2","pages":"135-151"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378758/pdf/","citationCount":"0","resultStr":"{\"title\":\"Sensitivity Analysis of <i>C. auris, S. cerevisiae</i>, and <i>C. cladosporioides</i> by Irradiation with Far-UVC, UVC, and UVB.\",\"authors\":\"Anna-Maria Gierke, Martin Hessling\",\"doi\":\"10.20411/pai.v9i2.723\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The World Health Organization has published a list of pathogenic fungi with prior-itizing groups and calls for research and development of antifungal measures, with <i>Candida auris</i> belonging to the group with high priority.</p><p><strong>Methods: </strong>The photosensitivity towards short wavelength ultraviolet irradiation (Far-UVC, UVC, and UVB) was investigated and compared to other yeasts (<i>Saccharomyces cerevisiae</i>) and a mold (<i>Cladosporium cladosporioides</i>). The observed 1-log reduction doses were compared to literature values of other representatives of the genus <i>Candida</i>, but also with <i>S. cerevisiae, Aspergillus niger,</i> and <i>A. fumigatus</i>.</p><p><strong>Results: </strong>For the determined 1-log reduction doses, an increase with higher wavelengths was observed. A 1-log reduction dose of 4.3 mJ/cm<sup>2</sup> was determined for <i>C. auris</i> when irradiated at 222 nm, a dose of 6.1 mJ/cm<sup>2</sup> at 254 nm and a 1-log reduction dose of 51.3 mJ/cm<sup>2</sup> was required when irradiated with UVB.</p><p><strong>Conclusions: </strong>It was observed that <i>S. cerevisiae</i> is a possible surrogate for <i>C. auris</i> for irradiation with Far-UVC and UVB due to close 1-log reduction doses. No surrogate suitability was verified for <i>C. cladosporioides</i> in relation to <i>A. niger</i> and <i>A. fumigatus</i> for irradiation with a wavelength of 254 nm and for <i>A. niger</i> at 222 nm.</p>\",\"PeriodicalId\":36419,\"journal\":{\"name\":\"Pathogens and Immunity\",\"volume\":\"9 2\",\"pages\":\"135-151\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378758/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathogens and Immunity\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.20411/pai.v9i2.723\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathogens and Immunity","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20411/pai.v9i2.723","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
背景：世界卫生组织公布了一份致病真菌优先群体名单，呼吁研究和开发抗真菌措施，白色念珠菌属于优先群体：方法：研究了白色念珠菌对短波紫外线照射（远紫外线、紫外线和紫外线）的光敏性，并与其他酵母菌（酿酒酵母）和霉菌（Cladosporium cladosporioides）进行了比较。观察到的 1-log降低剂量与其他念珠菌属代表菌种的文献值进行了比较，同时也与酿酒酵母、黑曲霉和烟曲霉进行了比较：结果：在确定的 1-log还原剂量中，观察到波长越高，剂量越大。在 222 纳米波长下照射 C. auris，1-log 减少剂量为 4.3 mJ/cm2；在 254 纳米波长下照射 C. auris，1-log 减少剂量为 6.1 mJ/cm2；在 UVB 波长下照射 C. auris，1-log 减少剂量为 51.3 mJ/cm2：结论：研究发现，由于远紫外和紫外线的1-log减少剂量接近，谷氨酸酵母菌可能是用远紫外和紫外线照射C.在波长为 254 纳米和 222 纳米的辐照条件下，C. cladosporioides 与 A. niger 和 A. fumigatus 的代用性未得到验证。
Sensitivity Analysis of C. auris, S. cerevisiae, and C. cladosporioides by Irradiation with Far-UVC, UVC, and UVB.
Background: The World Health Organization has published a list of pathogenic fungi with prior-itizing groups and calls for research and development of antifungal measures, with Candida auris belonging to the group with high priority.
Methods: The photosensitivity towards short wavelength ultraviolet irradiation (Far-UVC, UVC, and UVB) was investigated and compared to other yeasts (Saccharomyces cerevisiae) and a mold (Cladosporium cladosporioides). The observed 1-log reduction doses were compared to literature values of other representatives of the genus Candida, but also with S. cerevisiae, Aspergillus niger, and A. fumigatus.
Results: For the determined 1-log reduction doses, an increase with higher wavelengths was observed. A 1-log reduction dose of 4.3 mJ/cm2 was determined for C. auris when irradiated at 222 nm, a dose of 6.1 mJ/cm2 at 254 nm and a 1-log reduction dose of 51.3 mJ/cm2 was required when irradiated with UVB.
Conclusions: It was observed that S. cerevisiae is a possible surrogate for C. auris for irradiation with Far-UVC and UVB due to close 1-log reduction doses. No surrogate suitability was verified for C. cladosporioides in relation to A. niger and A. fumigatus for irradiation with a wavelength of 254 nm and for A. niger at 222 nm.
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