在大鼠麻醉实验中获取更佳原始大脑信号的有效方法。

IF 1.3 4区 医学 Q4 PHARMACOLOGY & PHARMACY Journal of pharmacological and toxicological methods Pub Date : 2024-09-01 DOI:10.1016/j.vascn.2024.107551
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引用次数: 0

摘要

本文介绍了一种进行大鼠麻醉实验的高效方法,旨在提高获得的原始脑信号的质量。所提出的方法能在实验过程中获取动物大脑信号,而不受肌肉噪声的干扰。首先,将α-氯糖(a-c)与异氟烷结合使用,诱导大鼠麻醉。随后,使用 Dexdomitor 来防止大鼠在采集大脑信号时发生肌肉运动,从而进一步提高信号质量。实验结果最终证明,我们的麻醉方法能产生更纯净的原始信号,并在数据采集过程中表现出更高的鲁棒性,优于仅依赖异氟醚或氯胺酮-恶嗪组合的现有方法。值得注意的是,这种性能的提高是在对重要生理参数(包括体温、呼吸和心率)影响最小的情况下实现的。此外,与异氟醚持续给药或氯胺酮-恶嗪 30 分钟窗口期所能达到的较短麻醉时间相比,a-c 能有效维持长达 7 小时的麻醉,这凸显了我们所建议方法的实用优势。最后,实验后的观察证实,动物的行为逐渐恢复正常,没有任何痛苦或不良反应的迹象,这表明我们的方法既有效又安全。
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The efficient method to get better raw brain signal on rat anesthetics experiment

This paper introduces an efficient methodology for conducting rat anesthesia experiments, aimed at enhancing the quality of raw brain signals obtained. The proposed approach enables the acquisition of animal brain signals during experiments without the confounding influence of muscle noise. Initially, the use of alpha-chloralose (a-c) in conjunction with Isoflurane is introduced to induce anesthesia in rats. Subsequently, Dexdomitor is administered to prevent muscular movements during the collection of brain signals, further refining the signal quality. Experimental outcomes conclusively demonstrate that our anesthesia method produces cleaner raw signals and exhibits improved robustness during data acquisition, outperforming existing methods that rely solely on Isoflurane or the Ketamine-Xylazine combination. Notably, this improved performance is achieved with minimal alterations to vital physiological parameters, including body temperature, respiration, and heart rates. Moreover, the efficacy of a-c in maintaining anesthesia for up to 7 h stands in contrast to the shorter durations achievable with continuous Isoflurane administration or the 30-min window offered by Ketamine-Xylazine, highlighting the practical advantages of our proposed method. Finally, post-experiment observations confirmed that the animals gradually returned to normal behavior without any signs of distress or adverse effects, indicating that our method was both effective and safe.

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来源期刊
Journal of pharmacological and toxicological methods
Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY
CiteScore
3.60
自引率
10.50%
发文量
56
审稿时长
26 days
期刊介绍: Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.
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