半枝莲提取物的细胞毒性、抗氧化性和酶抑制活性及其 HPLC-ESI-QTOF-MS 图谱:洞察一种尚未开发的半枝莲物种

IF 2.5 3区 医学 Q3 CHEMISTRY, MEDICINAL Fitoterapia Pub Date : 2024-09-08 DOI:10.1016/j.fitote.2024.106207
Gokhan Zengin , Álvaro Fernández-Ochoa , María de la Luz Cádiz-Gurrea , Francisco-Javier Leyva Jiménez , Abdullahi Ibrahim Uba , Gunes Ak , Sanam Iram Soomro , Maruf Balos , Ugur Cakilcioglu , Maria João Rodrigues , Catarina G. Pereira , Luísa Custódio
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引用次数: 0

摘要

矢车菊属植物因其生物潜力而在制药和保健品领域备受关注。基于这些信息,我们旨在评估一种未研究物种--矢车菊(Centaurea stapfiana)提取物的生物特性(抗氧化、酶抑制和细胞毒性)和化学特征。乙醇/水提取物中的总酚含量最高,为 32.17 毫克 GAE/克。通过 HPLC-ESI-QTOF-MS 分析,共鉴定出 102 种酚类化合物。这些化合物主要是羟基苯甲酸、羟基肉桂酸和黄酮类化合物。在抗氧化试验中,乙醇/水提取物的自由基清除和还原能力最强。不过,在酶抑制试验中,乙醇提取物的活性最高。提取物还在两种肿瘤细胞系(RAW 264.7 和 HepG2)和一种非肿瘤细胞系(S17)上进行了测试。乙醇提取物显示出对 HepG2 的良好效果(细胞存活率:50 克/毫升时为 28.6%)。此外,我们还研究了化合物与酶和细胞靶标之间的相互作用。结果发现,槲皮素-3-木糖基-(1- > 6)-葡萄糖苷与 iNOS 之间存在良好的相互作用。总之,我们的研究结果表明,C. stapfiana 可被视为一种多功能原料,用于开发医药和化妆品领域中促进健康的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Cytotoxic, antioxidant, and enzyme inhibitory activities of Centaurea stapfiana extracts and their HPLC-ESI-QTOF-MS profiles: Insights into an unexplored Centaurea species

The members of the genus Centaurea have a great interest in pharmaceutical and nutraceutical fields due to their biological potential. Based on this information, we aimed to evaluate the biological properties (antioxidant, enzyme inhibition and cytotoxicity) and chemical profile of the extract of Centaurea stapfiana, an unstudied species. The highest total phenolic content was found in the ethanol/water extract with 32.17 mg GAE/g. A total of 102 of them were identified by HPLC-ESI-QTOF-MS analysis. These compounds were mainly hydroxybenzoic acid and hydroxycinnamic acid as well as flavonoids. In the antioxidant tests, the ethanol/water extract had the best free radical scavenging and reducing ability. However, in the enzyme inhibition test, the ethanol extract was the most active. The extracts were also tested on two tumour cell lines (RAW 264.7 and HepG2) and one non-tumour cell line (S17). The ethanol extract showed the promising effect on HepG2 (cell viability: 28.6 % at 50 g/ml). Furthermore, we examined the interactions between the compounds and enzymatic and cellular targets. A good interaction was found between quercetin-3-xylosyl-(1- > 6)-glucoside and iNOS. In summary, our results suggest that C. stapfiana can be considered as a versatile raw material for the development of health-promoting applications in the pharmaceutical and cosmeceutical fields.

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来源期刊
Fitoterapia
Fitoterapia 医学-药学
CiteScore
5.80
自引率
2.90%
发文量
198
审稿时长
1.5 months
期刊介绍: Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.
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