{"title":"LC-MS/MS-QTOF分析Anodendron parviflorum（Roxb.）叶提取物并探索其抗氧化、抗菌和细胞毒性潜力","authors":"Arunagiri Sharmila, Chinnadurai Immanuel Selvaraj","doi":"10.1186/s43094-024-00695-1","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Traditional medicine claims that <i>Anodendron parviflorum</i> has benefits for treating various human diseases. The present study seeks to understand better the phytochemical and LC–MS/MS-QTOF profiling of <i>A. parviflorum</i>'s ethanolic extract and to investigate the properties of the different solvents of <i>A. parviflorum</i> for anti-inflammatory, antioxidant, antimicrobial, toxicity, and cytotoxic effects.</p><h3>Results</h3><p>The quantitative methods exhibited higher total phenolics (327.16 ± 2.4 mg GAE/g dw), total flavonoid (109.82 ± 1.9 mg QE/g dw), and total alkaloid (14.13 ± 0.09%) content in ethanol extract. In contrast, a higher total extraction value (22.8 ± 0.6%) and total terpenoid (57.23 ± 0.06 mg LL/g dw) content was shown in the methanol extract of <i>A. parviflorum</i>. LC–MS/MS-QTOF analysis of its ethanolic extract revealed a notable occurrence of phenols and flavonoids. The ethanolic extract of <i>A. parviflorum</i> exhibited significant antioxidant activities with lower IC<sub>50</sub> values in DPPH, phosphomolybdenum, and metal chelating and reducing power assay. The methanolic extract of <i>A. parviflorum</i> had the more significant anti-inflammatory property (94.55 ± 0.1%) in the bovine serum albumin assay. The extracts also demonstrated a higher inhibition zone against pathogenic bacteria. The ethanolic extract of <i>A. parviflorum</i> demonstrated substantial cytotoxicity against A549 cells.</p><h3>Conclusion</h3><p>Consequently, these findings validate the use of <i>A. parviflorum</i> in traditional medical practices due to its bioactive compounds, which may have potential therapeutic value in various biomedical applications.</p><h3>Highlights</h3>\n<ul>\n <li>\n <p><i>A. parviflorum</i> leaves extract showed the presence of significant levels of alkaloids, flavonoids, phenols, and terpenoids.</p>\n </li>\n <li>\n <p>LC–MS/MS-QTOF analysis revealed notable bioactive compounds of <i>A. parviflorum.</i></p>\n </li>\n <li>\n <p>The different extracts showed remarkable antioxidant and antimicrobial activities.</p>\n </li>\n <li>\n <p>The extracts exhibited less hemolytic and higher thrombolytic activities.</p>\n </li>\n <li>\n <p>It demonstrated significant cytotoxicity against A549 cell lines.</p>\n </li>\n </ul><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00695-1","citationCount":"0","resultStr":"{\"title\":\"LC–MS/MS-QTOF analysis of Anodendron parviflorum (Roxb.) leaves extract and exploring its antioxidant, antimicrobial, and cytotoxic potential\",\"authors\":\"Arunagiri Sharmila, Chinnadurai Immanuel Selvaraj\",\"doi\":\"10.1186/s43094-024-00695-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Traditional medicine claims that <i>Anodendron parviflorum</i> has benefits for treating various human diseases. The present study seeks to understand better the phytochemical and LC–MS/MS-QTOF profiling of <i>A. parviflorum</i>'s ethanolic extract and to investigate the properties of the different solvents of <i>A. parviflorum</i> for anti-inflammatory, antioxidant, antimicrobial, toxicity, and cytotoxic effects.</p><h3>Results</h3><p>The quantitative methods exhibited higher total phenolics (327.16 ± 2.4 mg GAE/g dw), total flavonoid (109.82 ± 1.9 mg QE/g dw), and total alkaloid (14.13 ± 0.09%) content in ethanol extract. In contrast, a higher total extraction value (22.8 ± 0.6%) and total terpenoid (57.23 ± 0.06 mg LL/g dw) content was shown in the methanol extract of <i>A. parviflorum</i>. LC–MS/MS-QTOF analysis of its ethanolic extract revealed a notable occurrence of phenols and flavonoids. The ethanolic extract of <i>A. parviflorum</i> exhibited significant antioxidant activities with lower IC<sub>50</sub> values in DPPH, phosphomolybdenum, and metal chelating and reducing power assay. The methanolic extract of <i>A. parviflorum</i> had the more significant anti-inflammatory property (94.55 ± 0.1%) in the bovine serum albumin assay. The extracts also demonstrated a higher inhibition zone against pathogenic bacteria. The ethanolic extract of <i>A. parviflorum</i> demonstrated substantial cytotoxicity against A549 cells.</p><h3>Conclusion</h3><p>Consequently, these findings validate the use of <i>A. parviflorum</i> in traditional medical practices due to its bioactive compounds, which may have potential therapeutic value in various biomedical applications.</p><h3>Highlights</h3>\\n<ul>\\n <li>\\n <p><i>A. parviflorum</i> leaves extract showed the presence of significant levels of alkaloids, flavonoids, phenols, and terpenoids.</p>\\n </li>\\n <li>\\n <p>LC–MS/MS-QTOF analysis revealed notable bioactive compounds of <i>A. parviflorum.</i></p>\\n </li>\\n <li>\\n <p>The different extracts showed remarkable antioxidant and antimicrobial activities.</p>\\n </li>\\n <li>\\n <p>The extracts exhibited less hemolytic and higher thrombolytic activities.</p>\\n </li>\\n <li>\\n <p>It demonstrated significant cytotoxicity against A549 cell lines.</p>\\n </li>\\n </ul><h3>Graphical abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":577,\"journal\":{\"name\":\"Future Journal of Pharmaceutical Sciences\",\"volume\":\"10 1\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00695-1\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s43094-024-00695-1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1186/s43094-024-00695-1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
LC–MS/MS-QTOF analysis of Anodendron parviflorum (Roxb.) leaves extract and exploring its antioxidant, antimicrobial, and cytotoxic potential
Background
Traditional medicine claims that Anodendron parviflorum has benefits for treating various human diseases. The present study seeks to understand better the phytochemical and LC–MS/MS-QTOF profiling of A. parviflorum's ethanolic extract and to investigate the properties of the different solvents of A. parviflorum for anti-inflammatory, antioxidant, antimicrobial, toxicity, and cytotoxic effects.
Results
The quantitative methods exhibited higher total phenolics (327.16 ± 2.4 mg GAE/g dw), total flavonoid (109.82 ± 1.9 mg QE/g dw), and total alkaloid (14.13 ± 0.09%) content in ethanol extract. In contrast, a higher total extraction value (22.8 ± 0.6%) and total terpenoid (57.23 ± 0.06 mg LL/g dw) content was shown in the methanol extract of A. parviflorum. LC–MS/MS-QTOF analysis of its ethanolic extract revealed a notable occurrence of phenols and flavonoids. The ethanolic extract of A. parviflorum exhibited significant antioxidant activities with lower IC50 values in DPPH, phosphomolybdenum, and metal chelating and reducing power assay. The methanolic extract of A. parviflorum had the more significant anti-inflammatory property (94.55 ± 0.1%) in the bovine serum albumin assay. The extracts also demonstrated a higher inhibition zone against pathogenic bacteria. The ethanolic extract of A. parviflorum demonstrated substantial cytotoxicity against A549 cells.
Conclusion
Consequently, these findings validate the use of A. parviflorum in traditional medical practices due to its bioactive compounds, which may have potential therapeutic value in various biomedical applications.
Highlights
A. parviflorum leaves extract showed the presence of significant levels of alkaloids, flavonoids, phenols, and terpenoids.
LC–MS/MS-QTOF analysis revealed notable bioactive compounds of A. parviflorum.
The different extracts showed remarkable antioxidant and antimicrobial activities.
The extracts exhibited less hemolytic and higher thrombolytic activities.
It demonstrated significant cytotoxicity against A549 cell lines.
期刊介绍：
Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.
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