通过 Chd1 和 FACT 分解转录诱导的六聚体-核小体复合物

IF 14.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Cell Pub Date : 2024-09-12 DOI:10.1016/j.molcel.2024.08.022
Maik Engeholm, Johann J. Roske, Elisa Oberbeckmann, Christian Dienemann, Michael Lidschreiber, Patrick Cramer, Lucas Farnung
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引用次数: 0

摘要

为了维持转录基因的核小体组织,依赖 ATP 的染色质重塑者与组蛋白伴侣合作。在这里,我们发现在酵母基因的 5′末端,RNA 聚合酶 II(RNAPII)会产生直接毗邻核小体的六聚体。产生的六聚体-核小体复合物随后被 Chd1 分解。我们展示了 Chd1 与六聚体-核小体复合物结合的两种冷冻电子显微镜(cryo-EM)结构,分别发生在通过 FACT 恢复缺失的内部 H2A/H2B 二聚体之前和之后。Chd1 与复合物独特地相互作用,定位其 ATPase 结构域,使六聚体远离核糖体。在缺乏内部 H2A/H2B 二聚体的情况下,其 DNA 结合结构域 (DBD) 会与 ATPase 结构域相抵触,这表明它处于抑制状态。通过 FACT 恢复二聚体会引发重排,从而移位 DBD 并刺激 Chd1 重塑。我们的研究结果证明了染色质重塑者如何与复杂的核小体组装相互作用,并表明 Chd1 和 FACT 如何共同支持 RNAPII 的转录。
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Resolution of transcription-induced hexasome-nucleosome complexes by Chd1 and FACT

To maintain the nucleosome organization of transcribed genes, ATP-dependent chromatin remodelers collaborate with histone chaperones. Here, we show that at the 5′ ends of yeast genes, RNA polymerase II (RNAPII) generates hexasomes that occur directly adjacent to nucleosomes. The resulting hexasome-nucleosome complexes are then resolved by Chd1. We present two cryoelectron microscopy (cryo-EM) structures of Chd1 bound to a hexasome-nucleosome complex before and after restoration of the missing inner H2A/H2B dimer by FACT. Chd1 uniquely interacts with the complex, positioning its ATPase domain to shift the hexasome away from the nucleosome. In the absence of the inner H2A/H2B dimer, its DNA-binding domain (DBD) packs against the ATPase domain, suggesting an inhibited state. Restoration of the dimer by FACT triggers a rearrangement that displaces the DBD and stimulates Chd1 remodeling. Our results demonstrate how chromatin remodelers interact with a complex nucleosome assembly and suggest how Chd1 and FACT jointly support transcription by RNAPII.

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来源期刊
Molecular Cell
Molecular Cell 生物-生化与分子生物学
CiteScore
26.00
自引率
3.80%
发文量
389
审稿时长
1 months
期刊介绍: Molecular Cell is a companion to Cell, the leading journal of biology and the highest-impact journal in the world. Launched in December 1997 and published monthly. Molecular Cell is dedicated to publishing cutting-edge research in molecular biology, focusing on fundamental cellular processes. The journal encompasses a wide range of topics, including DNA replication, recombination, and repair; Chromatin biology and genome organization; Transcription; RNA processing and decay; Non-coding RNA function; Translation; Protein folding, modification, and quality control; Signal transduction pathways; Cell cycle and checkpoints; Cell death; Autophagy; Metabolism.
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