Katherine M Widmer, Faith Rahic-Seggerman, Ahlea Forster, Amanda Ahrens-Kress, Mary Sauer, Shankumar Mooyottu, Akhil Vinithakumari, Aaron Dunkerson-Kurzhumov, Brett Sponseller, Matti Kiupel, Stephan Schmitz-Esser, Christopher K Tuggle
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The hypothesis was that an increase in complexity of microbiota inoculum will allow for more stability in the composition of the gut microbiota of SCID piglets. This was tested across multiple litters of SCID piglets with three different defined microbiota consortium (2-strain, 6-strain, 7-strain). All piglets received their designated defined microbiota by oral gavage immediately after birth and again 24 hours later. There was no effect of SCID genotype on the composition of the gut microbiota, but there was a significant effect due to piglet age. Additionally, all three defined microbiota consortia were deemed safe to use in SCID piglets, and the 7-strain microbiota was the most stable over time. Based on these results, the 7-strain defined microbiota will be added to the SCID pig husbandry protocol, allowing for a more reproducible model.","PeriodicalId":501246,"journal":{"name":"bioRxiv - Genetics","volume":"109 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Genotype and Age on a Defined Microbiota in Gnotobiotic SCID Piglets\",\"authors\":\"Katherine M Widmer, Faith Rahic-Seggerman, Ahlea Forster, Amanda Ahrens-Kress, Mary Sauer, Shankumar Mooyottu, Akhil Vinithakumari, Aaron Dunkerson-Kurzhumov, Brett Sponseller, Matti Kiupel, Stephan Schmitz-Esser, Christopher K Tuggle\",\"doi\":\"10.1101/2024.09.03.611011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Severe combined immunodeficient (SCID) individuals lack functional T and B lymphocytes, leading to a deficient adaptive immune system. 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引用次数: 0
摘要
严重联合免疫缺陷(SCID)个体缺乏功能性 T 淋巴细胞和 B 淋巴细胞,导致适应性免疫系统缺陷。SCID 猪是一种独特的大型动物生物医学模型,因为它们与人类有许多相似之处,可以收集再生医学、癌症和其他生物医学研究课题的可转化数据。许多研究表明,早期肠道微生物群的发育对肠道屏障和免疫系统的发育十分必要,但这些动物通常是剖腹产,正常的肠道微生物菌群尚未覆盖。我们的假设是,增加微生物群接种物的复杂性将使 SCID 仔猪肠道微生物群的组成更加稳定。我们在多窝 SCID 仔猪中使用三种不同的定义微生物群(2-菌株、6-菌株、7-菌株)进行了测试。所有仔猪在出生后立即口服指定的微生物群,24 小时后再次口服。SCID 基因型对肠道微生物群的组成没有影响,但仔猪年龄有显著影响。此外,在 SCID 仔猪中使用所有三种确定的微生物群都被认为是安全的,而 7 菌株微生物群随着时间的推移最为稳定。基于这些结果,7 种菌株定义的微生物群将被添加到 SCID 仔猪饲养方案中,从而建立一个可重复性更强的模型。
Effect of Genotype and Age on a Defined Microbiota in Gnotobiotic SCID Piglets
Severe combined immunodeficient (SCID) individuals lack functional T and B lymphocytes, leading to a deficient adaptive immune system. SCID pigs are a unique large animal biomedical model as they possess many similarities to humans, allowing for the collection of translatable data in regenerative medicine, cancer, and other biomedical research topics. While many studies suggest early gut microbiota development is necessary for developing the intestinal barrier and immune system, these animals are often cesarian section derived, leaving them uncolonized for normal intestinal microflora. The hypothesis was that an increase in complexity of microbiota inoculum will allow for more stability in the composition of the gut microbiota of SCID piglets. This was tested across multiple litters of SCID piglets with three different defined microbiota consortium (2-strain, 6-strain, 7-strain). All piglets received their designated defined microbiota by oral gavage immediately after birth and again 24 hours later. There was no effect of SCID genotype on the composition of the gut microbiota, but there was a significant effect due to piglet age. Additionally, all three defined microbiota consortia were deemed safe to use in SCID piglets, and the 7-strain microbiota was the most stable over time. Based on these results, the 7-strain defined microbiota will be added to the SCID pig husbandry protocol, allowing for a more reproducible model.