GhPME36 通过影响棉花叶片细胞壁的生物合成,加剧棉花对 Liriomyza sativae 的易感性

IF 4.4 1区 生物学 Q1 BIOLOGY BMC Biology Pub Date : 2024-09-11 DOI:10.1186/s12915-024-01999-7
Zheng Yang, Menglei Wang, Senmiao Fan, Zhen Zhang, Doudou Zhang, Jie He, Tongyi Li, Renhui Wei, Panpan Wang, Muhammad Dawood, Weijie Li, Lin Wang, Shaogan Wang, Youlu Yuan, Haihong Shang
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引用次数: 0

摘要

棉花是一种重要的经济作物,也是 Liriomyza sativae 的寄主。果胶甲基酯酶(PME)介导的果胶代谢在植物体内的多种生物过程中发挥着不可或缺的作用。然而,果胶甲基酯酶的多效性功能往往会对作物的抗虫性产生难以预测的影响。此外,PME 是否以及如何影响对 Liriomyza sativae 的易感性仍不清楚。在这里,我们从陆地棉(Gossypium hirsutum L.)中分离出了位于细胞壁中的 GhPME36。有趣的是,在棉花中过表达 GhPME36 会导致对拟南芥(Liriomyza sativae)的严重易感性,但会增加拟南芥的叶片生物量。细胞学观察显示,与 WT 叶片相比,GhPME36-OE 棉花叶片的细胞壁更薄,含有更多的脱甲基酯化果胶,而 GhPME36-OE 棉花叶片细胞壁的可溶性糖含量相应更高;这两个因素都吸引了 Liriomyza sativae 以 GhPME36-OE 棉花叶片为食。代谢组分析表明,葡萄糖的积累存在显著差异。转录组分析进一步发现,当 GhPME36 过表达时,葡萄糖代谢途径中的 DEGs 丰富,这表明 GhPME36 通过影响细胞壁生物合成的结构和成分,加剧了对 Liriomyza sativae 的易感性。此外,GhPME36 与另一种果胶修饰酶 GhC/VIF1 相互作用,以维持果胶甲酯化的动态稳定性。综上所述,我们的研究结果揭示了 GhPME36 加剧对 Liriomyza sativae 的易感性的细胞学和分子机制。这项研究拓宽了人们对 PME 功能的认识,并为植物对害虫的抗性和转基因植物的安全性提供了新的见解。
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GhPME36 aggravates susceptibility to Liriomyza sativae by affecting cell wall biosynthesis in cotton leaves
Cotton is an important economic crop and a host of Liriomyza sativae. Pectin methylesterase (PME)-mediated pectin metabolism plays an indispensable role in multiple biological processes in planta. However, the pleiotropic functions of PME often lead to unpredictable effects on crop resistance to pests. Additionally, whether and how PME affects susceptibility to Liriomyza sativae remain unclear. Here, we isolated GhPME36, which is located in the cell wall, from upland cotton (Gossypium hirsutum L.). Interestingly, the overexpression of GhPME36 in cotton caused severe susceptibility to Liriomyza sativae but increased leaf biomass in Arabidopsis. Cytological observations revealed that the cell wall was thinner with more demethylesterified pectins in GhPME36-OE cotton leaves than in WT leaves, whereas the soluble sugar content of GhPME36-OE cotton leaf cell walls was accordingly higher; both factors attracted Liriomyza sativae to feed on GhPME36-OE cotton leaves. Metabolomic analysis demonstrated that glucose was significantly differentially accumulated. Transcriptomic analysis further revealed DEGs enriched in glucose metabolic pathways when GhPME36 was overexpressed, suggesting that GhPME36 aggravates susceptibility to Liriomyza sativae by affecting both the structure and components of cell wall biosynthesis. Moreover, GhPME36 interacts with another pectin-modifying enzyme, GhC/VIF1, to maintain the dynamic stability of pectin methyl esterification. Taken together, our results reveal the cytological and molecular mechanisms by which GhPME36 aggravates susceptibility to Liriomyza sativae. This study broadens the knowledge of PME function and provides new insights into plant resistance to pests and the safety of genetically modified plants.
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来源期刊
BMC Biology
BMC Biology 生物-生物学
CiteScore
7.80
自引率
1.90%
发文量
260
审稿时长
3 months
期刊介绍: BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.
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