在清洗水的深处:从新鲜水果加工厂分离机会性细菌

IF 3.3 3区 医学 Q2 MICROBIOLOGY Pathogens Pub Date : 2024-09-06 DOI:10.3390/pathogens13090768
Piotr Kanarek, Barbara Breza-Boruta, Tomasz Bogiel
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引用次数: 0

摘要

水果和蔬菜采后加工厂的特点是大量消耗水资源。清洗水可能成为微生物周期性或永久性栖息的环境,特别是在水槽和冲洗通道内壁形成生物膜的情况下。尽管许多国家都实施了综合食品安全监控系统,但食源性病原体仍然是全球公共卫生和食品安全的一个问题,尤其是蔬菜和水果等微加工食品。因此,有必要开展研究,探索清洗水的水质,以保护微加工食品免受食源性病原体的污染。因此,本研究旨在分离和鉴定污染四家鲜切加工厂(波兰)清洗水的细菌,并评估所选菌种的表型抗生素耐药性特征。细菌采用膜过滤法进行分离,并通过质谱法进行鉴定,然后根据欧盟食品安全委员会(EUCAST)的指导原则进行抗生素药敏试验。结果显示,水中总需氧细菌的污染水平从 1.30 × 106 cfu/mL 到 2.54 × 108 cfu/mL 不等。在分离的细菌中,发现了机会性病原体,包括粪肠球菌、铜绿假单胞菌、氧乐氏克雷伯菌、肺炎克雷伯菌、大肠沙雷氏菌和普通变形杆菌。特别值得注意的结果是发现了耐头孢吡肟的克雷伯菌分离株。这些发现凸显了监测微加工食品中微生物菌群的重要性,以及采用适当的卫生控制程序将病原体污染风险降至最低的必要性,从而确保产品在整个供应链中保持安全和高质量。
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In the Depths of Wash Water: Isolation of Opportunistic Bacteria from Fresh-Cut Processing Plants
The fruit and vegetable industry in post-harvest processing plants is characterized by a substantial consumption of water resources. Wash waters may serve as an environment for the periodic or permanent habitation of microorganisms, particularly if biofilm forms on the inner walls of tanks and flushing channels. Despite the implementation of integrated food safety monitoring systems in numerous countries, foodborne pathogens remain a global public health and food safety concern, particularly for minimally processed food products such as vegetables and fruits. This necessitates the importance of studies that will explore wash water quality to safeguard minimally processed food against foodborne pathogen contamination. Therefore, the current study aimed to isolate and identify bacteria contaminating the wash waters of four fresh-cut processing plants (Poland) and to evaluate the phenotypic antibiotic resistance profiles in selected species. Bacteria were isolated using membrane filtration and identified through mass spectrometry, followed by antibiotic susceptibility testing according to EUCAST guidelines. The results revealed that the level of contamination with total aerobic bacteria in the water ranged from 1.30 × 106 cfu/mL to 2.54 × 108 cfu/mL. Among the isolates, opportunistic pathogens including Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella oxytoca, Klebsiella pneumoniae, Serratia marcescens, and Proteus vulgaris strains were identified. An especially noteworthy result was the identification of cefepime-resistant K. oxytoca isolates. These findings highlight the importance of monitoring the microbial microflora in minimally processed foods and the need for appropriate sanitary control procedures to minimize the risk of pathogen contamination, ensuring that products remain safe and of high quality throughout the supply chain.
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来源期刊
Pathogens
Pathogens Medicine-Immunology and Allergy
CiteScore
6.40
自引率
8.10%
发文量
1285
审稿时长
17.75 days
期刊介绍: Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.
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