Michael A. Taylor, Jung Un Ally Choi, Shiva Muthuswamy, Marco A. Enriquez Martinez, Jan Lauko, Amanda W. Kijas and Alan E. Rowan
{"title":"利用共焦反射对三维细胞模型进行灵敏的无标记成像，将毒性降至最低","authors":"Michael A. Taylor, Jung Un Ally Choi, Shiva Muthuswamy, Marco A. Enriquez Martinez, Jan Lauko, Amanda W. Kijas and Alan E. Rowan","doi":"10.1039/D4BM00304G","DOIUrl":null,"url":null,"abstract":"<p >Confocal reflectance imaging typically suffers from high background and poor sensitivity. We demonstrate sensitive and low-background reflectance imaging of cells encapsulated in transparent 3D hydrogels. Nanoscale cell morphology is visualized with sensitivity similar to confocal fluorescence, with low laser power, minimal specimen preparation, and reduced toxicity.</p>","PeriodicalId":65,"journal":{"name":"Biomaterials Science","volume":" 22","pages":" 5722-5727"},"PeriodicalIF":5.8000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sensitive label free imaging of 3D cell models with minimal toxicity using confocal reflectance†\",\"authors\":\"Michael A. Taylor, Jung Un Ally Choi, Shiva Muthuswamy, Marco A. Enriquez Martinez, Jan Lauko, Amanda W. Kijas and Alan E. Rowan\",\"doi\":\"10.1039/D4BM00304G\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Confocal reflectance imaging typically suffers from high background and poor sensitivity. We demonstrate sensitive and low-background reflectance imaging of cells encapsulated in transparent 3D hydrogels. Nanoscale cell morphology is visualized with sensitivity similar to confocal fluorescence, with low laser power, minimal specimen preparation, and reduced toxicity.</p>\",\"PeriodicalId\":65,\"journal\":{\"name\":\"Biomaterials Science\",\"volume\":\" 22\",\"pages\":\" 5722-5727\"},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2024-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomaterials Science\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2024/bm/d4bm00304g\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomaterials Science","FirstCategoryId":"5","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/bm/d4bm00304g","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Sensitive label free imaging of 3D cell models with minimal toxicity using confocal reflectance†
Confocal reflectance imaging typically suffers from high background and poor sensitivity. We demonstrate sensitive and low-background reflectance imaging of cells encapsulated in transparent 3D hydrogels. Nanoscale cell morphology is visualized with sensitivity similar to confocal fluorescence, with low laser power, minimal specimen preparation, and reduced toxicity.
期刊介绍：
Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.
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