Anna Ścigaczewska, Tomasz Boruta, Weronika Grzesiak, Marcin Bizukojć
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A low nitrogen concentration of 1 g YE L− 1 led to the limitation of S. rimosus growth and, in consequence, the development of the smaller number of large pseudohyphal agglomerates (pellets) of projected area higher than 105 µm2 compared to the culture containing a high amount of nitrogen source. In both cases talc microparticles were embedded into pellets and caused the decrease in their sizes. The lower amount of talc (5 g TALC L− 1) usually caused the weaker effect on S. rimosus morphology and metabolite production than the higher one. This correlation between the microparticles effect on morphology and metabolism of S. rimosus was especially noticeable in the biosynthesis of oxytetracycline, 2-acetyl-2-dicarboxamide oxytetracycline (ADOTC) and spinoxazine A. Compared to the control run, in MPEC their levels increased 4-fold, 5-fold and 1.6-fold respectively. 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引用次数: 0
摘要
研究了滑石微粒在不同初始有机氮浓度下对菱形芽孢杆菌新陈代谢和形态的影响。摇瓶培养是在酵母提取物(氮源)浓度为 1 g YE L- 1 和 20 g YE L- 1 的培养基中进行的。在微粒子强化培养(MPEC)运行中测试了 5 g TALC L- 1 和 10 g TALC L- 1 两种浓度的滑石微粒子。20 g YE L- 1 的高浓度氮促进了投影面积小于 105 µm2 的小团聚体(颗粒)和分散的假菌丝的发育。1 g YE L- 1 的低氮浓度限制了 S. rimosus 的生长,因此,与含有大量氮源的培养物相比,投影面积大于 105 µm2 的大型假头状菌团(颗粒)的数量较少。在这两种情况下,滑石粉微粒都会嵌入颗粒中,并导致其体积减小。较低的滑石粉用量(5 g TALC L-1)通常比较高的滑石粉用量对菱形虫的形态和代谢物产生的影响要小。与对照组相比,在 MPEC 中,它们的含量分别增加了 4 倍、5 倍和 1.6 倍。添加滑石粉还能提高 2-甲硫基-顺式泽汀、龙葵酸 J 和米尔贝霉素 A3 的产量。
Analysis of secondary metabolites and morphology in Streptomyces rimosus microparticle-enhanced cultivation (MPEC) at various initial organic nitrogen concentrations
The influence of talc microparticles on metabolism and morphology of S. rimosus at various initial organic nitrogen concentrations was investigated. The shake flask cultivations were conducted in the media with yeast extract (nitrogen source) concentration equal to 1 g YE L− 1 and 20 g YE L− 1. Two talc microparticle concentrations of 5 g TALC L− 1 and 10 g TALC L− 1 were tested in microparticle-enhanced cultivation (MPEC) runs. A high nitrogen concentration of 20 g YE L− 1 promoted the development of small agglomerates (pellets) of projected area lower than 105 µm2 and dispersed pseudohyphae. A low nitrogen concentration of 1 g YE L− 1 led to the limitation of S. rimosus growth and, in consequence, the development of the smaller number of large pseudohyphal agglomerates (pellets) of projected area higher than 105 µm2 compared to the culture containing a high amount of nitrogen source. In both cases talc microparticles were embedded into pellets and caused the decrease in their sizes. The lower amount of talc (5 g TALC L− 1) usually caused the weaker effect on S. rimosus morphology and metabolite production than the higher one. This correlation between the microparticles effect on morphology and metabolism of S. rimosus was especially noticeable in the biosynthesis of oxytetracycline, 2-acetyl-2-dicarboxamide oxytetracycline (ADOTC) and spinoxazine A. Compared to the control run, in MPEC their levels increased 4-fold, 5-fold and 1.6-fold respectively. The addition of talc also improved the production of 2-methylthio-cis-zeatin, lorneic acid J and milbemycin A3.
期刊介绍:
Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology.
The journal is divided into the following editorial sections:
-Metabolic engineering
-Synthetic biology
-Whole-cell biocatalysis
-Microbial regulations
-Recombinant protein production/bioprocessing
-Production of natural compounds
-Systems biology of cell factories
-Microbial production processes
-Cell-free systems