病虫害甜樱桃树(Prunus avium L.)和健康甜樱桃树(Prunus avium L.)微生物群落的比较分析

IF 4.1 2区 生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2024-09-05 DOI:10.3390/microorganisms12091837
Tong Zhou, Xiaojuan Huang, Danyang Zhu, Yan Tang, Hongli Xu, Fanrong Ran, Hasin Ullah, Jiangli Tan
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引用次数: 0

摘要

欧洲甜樱桃(Prunus avium (L.))是蔷薇科植物,是最受欢迎、最具经济价值的水果之一。然而,流胶病的迅速蔓延和不良的管理方法已成为其生产的主要障碍。为了确定造成胶蛆病的病原微生物,我们在中国陕西省西安市对遭受严重胶蛆病和角甲虫危害的白鹿原果园和仅遭受胶蛆病危害的马峪河果园进行了观察比较。研究人员从健康组织和龈病组织中获取样本,利用 Illumina 16S rRNA 序列和内部转录间隔区(ITS)来鉴定这些样本中的细菌和真菌群落。α多样性分析表明,在牙龈病期间,疾病组织的真菌多样性明显高于健康组织。结果表明,真菌属的不平衡可能与牙龈病有关。物种相对分析表明,在牙龈肿病的病变组织中存在一些细菌属(Pelagibacterium、Halomonas、Azospirillum、Aquabacterium 和 Alistipes)和真菌属(Penicillium、Alternaria 和 Rhodotorula)。其中,细菌基因 Halomonas、Pelagibacterium、Chelativorans、Pantoea、Aquabacterium、Alternaria 和真菌基因 Penicillium、Cystobasidium、Rhodotorula 的相对丰度增加可能与阿维菌的牙龈病有关。细菌属 Methylobacterium、Psychroglaciecola、Aeromonas、Conexibacter 和真菌属 Didymella、Aureobasidium、Mycosphaerella、Meyerozyma 可能是牙龈病病原体的拮抗剂。这些发现是确定该疾病生物防治潜在候选者的第一步。
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Comparative Analysis of Microbial Communities in Diseased and Healthy Sweet Cherry Trees (Prunus avium L.)
The European sweet cherry Prunus avium (L.), a member of the Rosaceae family, is one of the most popular and economically valuable fruits. However, the rapid spread of gummosis and poor management practices have become the major obstacles to their production. To identify pathogenic microorganisms responsible for gummosis disease, we conducted observations comparing the garden of Bailuyuan, which heavily suffered from gummosis disease and horn beetle damage, with the orchard of Mayuhe, which only suffered from gummosis disease, both from Xi’an, Shaanxi, China. Samples were obtained from the healthy tissues and gummosis disease tissues that used the Illumina sequence of 16S rRNA and the internal transcribed spacer region (ITS) to identify bacterial and fungal communities in these samples. An alpha diversity analysis revealed a significantly higher fungal diversity of disease than in healthy tissue in the gummosis period. The results suggested that an imbalance in the fungal genera may be associated with gummosis disease. Species relative analyses showed some bacterial genera (Pelagibacterium, Halomonas, Azospirillum, Aquabacterium and Alistipes) and fungal genera (Penicillium, Alternaria and Rhodotorula) in the diseased tissues of gummosis. Among these, the increased relative abundance of the bacteria genes Halomonas, Pelagibacterium, Chelativorans, Pantoea, Aquabacterium, Alternaria and fungi genes Penicillium, Cystobasidium, Rhodotorula may be associated with gummosis of P. avium. The bacterial genera Methylobacterium, Psychroglaciecola, Aeromonas, Conexibacter and fungal genera Didymella, Aureobasidium, Mycosphaerella, Meyerozyma are probably antagonists of the pathogen of gummosis. These findings are an initial step in the identification of potential candidates for the biological control of the disease.
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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