使用胶状电极电化学板可视化分离电化学活性细菌

IF 7.4 Q1 ENGINEERING, ENVIRONMENTAL ACS ES&T engineering Pub Date : 2024-08-30 DOI:10.1021/acsestengg.4c00308
Bo Cao, Yuxuan Zang, Beizhen Xie, Ting Zhao, Hongyu Zhao, Yanhong Ge, Hong Liu, Yue Yi
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引用次数: 0

摘要

电化学活性细菌(EAB)是微生物电生和电合成的催化剂,在废水处理和生化工程中具有广阔的应用前景。然而,电化学活性菌的分离十分困难,目前仅有少数纯培养的电化学活性菌被报道。本研究建立了一种基于电化学板的新型胶状电极,首次实现了对 EAB 菌株的选择性筛选和可见菌落的形成。利用该方法分离出了一种新的 EAB--庆生红球菌(Rhodococcus qingshengii C6)。R. qingshengii C6能在多种底物光谱下发电,并能通过消耗电能还原常见的电子受体。初步研究了双向胞外电子传递机制,发现外源氧化还原介质在胞外电子传递中起着重要作用。该研究为 EAB 的分离和纯化提供了一种简便易行的方法,有助于宝贵细菌资源的发掘。
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Visualization Isolation of Electrochemically Active Bacteria by Using a Gelled Electrode-Based Electrochemical Plate
Electrochemically active bacteria (EAB) are catalysts of microbial electrogenesis and electrosynthesis, showing great prospects in wastewater treatment and biochemical engineering. However, isolating EAB is difficult, and only a few pure-cultured EAB have been reported. In this study, a novel gelled electrode based on an electrochemical plate is established, which for the first time realizes the selective screening of EAB strains and visible colony formation. A new EAB, Rhodococcus qingshengii C6, is isolated by using the method. R. qingshengii C6 is capable of electricity generation with a wide range of substrate spectra and is capable of reducing common electron acceptors by consuming electricity. The bidirectional extracellular electron transfer mechanism is preliminarily investigated, and exogenous redox mediators play an important role in extracellular electron transfer. This study provides an easy and simple method for the isolation and purification of EAB, contributing to the valuable bacterial resources exploration.
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来源期刊
ACS ES&T engineering
ACS ES&T engineering ENGINEERING, ENVIRONMENTAL-
CiteScore
8.50
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期刊介绍: ACS ES&T Engineering publishes impactful research and review articles across all realms of environmental technology and engineering, employing a rigorous peer-review process. As a specialized journal, it aims to provide an international platform for research and innovation, inviting contributions on materials technologies, processes, data analytics, and engineering systems that can effectively manage, protect, and remediate air, water, and soil quality, as well as treat wastes and recover resources. The journal encourages research that supports informed decision-making within complex engineered systems and is grounded in mechanistic science and analytics, describing intricate environmental engineering systems. It considers papers presenting novel advancements, spanning from laboratory discovery to field-based application. However, case or demonstration studies lacking significant scientific advancements and technological innovations are not within its scope. Contributions containing experimental and/or theoretical methods, rooted in engineering principles and integrated with knowledge from other disciplines, are welcomed.
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