{"title":"基于系统发生组学结合形态计量学、代谢组学和毒力数据的 Metarhizium anisopliae 物种群综合分类法","authors":"Noppol Kobmoo, Suchada Mongkolsamrit, Artit Khonsanit, Marjorie Cedeño-Sanchez, Nuntanat Arnamnart, Wasana Noisripoom, Papichaya Kwantong, Chutima Sonthirod, Wirulda Pootakham, Alongkorn Amnuaykanjanasin, Esteban Charria-Girón, Marc Stadler, Janet Jennifer Luangsa-ard","doi":"10.1186/s43008-024-00154-9","DOIUrl":null,"url":null,"abstract":"Metarhizium anisopliae (Clavicipitaceae, Hypocreales) is a globally distributed entomopathogenic fungus, which has been largely studied and used in agriculture for its potent entomopathogenicity. Since its taxonomic establishment as a member of Metarhizium, many closely related taxa have been described with highly similar morphology (cryptic species). A holotype specimen of M. anisopliae is not extant, and the ex-neotype strain (CBS 130.71) does not form a monophyletic clade with other strains, up to now, recognized as M. anisopliae sensu stricto. In this study, we have conducted an integrative taxonomic treatment of M. anisopliae sensu lato by including the ex-neotype strain of M. anisopliae, other unknown strains from our collections identified as M. anisopliae s. lat., as well as other known species that have been previously delimited as closely related but distinct to M. anisopliae. By including whole-genome sequencing, morphometric analysis, LC–MS based metabolomics, and virulence assays, we have demonstrated that M. anisopliae s. str. should also include M. lepidiotae (synonym), and that M. anisopliae s. str. differentiates from the other species of the complex by its metabolome and less severe entomopathogenicity. New taxa, namely M. hybridum, M. neoanisopliae and M. parapingshaense spp. nov., are proposed. The novel taxa proposed here have strong phylogenomics support, corroborated by fine-scale differences in the length/width of conidia/phialides, while the metabolomics and virulence data still largely overlap. We have also demonstrated via population genomics data the existence of local clonal lineages, particularly the one corresponding to the persistence of a biocontrol candidate strain that has been used in the field application for three years. This study showcases the utility of combining various data sources for accurate delimitation of species within an important group of fungal biocontrol agents against pest insects.","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":null,"pages":null},"PeriodicalIF":5.2000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrative taxonomy of Metarhizium anisopliae species complex, based on phylogenomics combined with morphometrics, metabolomics, and virulence data\",\"authors\":\"Noppol Kobmoo, Suchada Mongkolsamrit, Artit Khonsanit, Marjorie Cedeño-Sanchez, Nuntanat Arnamnart, Wasana Noisripoom, Papichaya Kwantong, Chutima Sonthirod, Wirulda Pootakham, Alongkorn Amnuaykanjanasin, Esteban Charria-Girón, Marc Stadler, Janet Jennifer Luangsa-ard\",\"doi\":\"10.1186/s43008-024-00154-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Metarhizium anisopliae (Clavicipitaceae, Hypocreales) is a globally distributed entomopathogenic fungus, which has been largely studied and used in agriculture for its potent entomopathogenicity. Since its taxonomic establishment as a member of Metarhizium, many closely related taxa have been described with highly similar morphology (cryptic species). A holotype specimen of M. anisopliae is not extant, and the ex-neotype strain (CBS 130.71) does not form a monophyletic clade with other strains, up to now, recognized as M. anisopliae sensu stricto. In this study, we have conducted an integrative taxonomic treatment of M. anisopliae sensu lato by including the ex-neotype strain of M. anisopliae, other unknown strains from our collections identified as M. anisopliae s. lat., as well as other known species that have been previously delimited as closely related but distinct to M. anisopliae. By including whole-genome sequencing, morphometric analysis, LC–MS based metabolomics, and virulence assays, we have demonstrated that M. anisopliae s. str. should also include M. lepidiotae (synonym), and that M. anisopliae s. str. differentiates from the other species of the complex by its metabolome and less severe entomopathogenicity. New taxa, namely M. hybridum, M. neoanisopliae and M. parapingshaense spp. nov., are proposed. The novel taxa proposed here have strong phylogenomics support, corroborated by fine-scale differences in the length/width of conidia/phialides, while the metabolomics and virulence data still largely overlap. We have also demonstrated via population genomics data the existence of local clonal lineages, particularly the one corresponding to the persistence of a biocontrol candidate strain that has been used in the field application for three years. This study showcases the utility of combining various data sources for accurate delimitation of species within an important group of fungal biocontrol agents against pest insects.\",\"PeriodicalId\":54345,\"journal\":{\"name\":\"Ima Fungus\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2024-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ima Fungus\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s43008-024-00154-9\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ima Fungus","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s43008-024-00154-9","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
拟黑僵菌(Metarhizium anisopliae,Clavicipitaceae,Hypocreales)是一种分布于全球的昆虫病原真菌,因其强大的昆虫致病性而被广泛研究并用于农业。自其在分类学上被确定为 Metarhizium 的一员以来,许多形态高度相似的近缘类群(隐种)已被描述。Anisopliae 的主模式标本并不存在,而前新模式菌株(CBS 130.71)也没有与其他菌株形成一个单系支系,到目前为止,这些菌株都被认定为严格意义上的 M. anisopliae。在本研究中,我们对 M. anisopliae sensu lato 进行了综合分类学处理,包括 M. anisopliae 的前原型菌株、从我们的藏品中鉴定为 M. anisopliae s. lat.的其他未知菌株,以及以前被划分为与 M. anisopliae 密切相关但又不同的其他已知物种。通过全基因组测序、形态计量分析、基于 LC-MS 的代谢组学和毒力测定,我们证明 M. anisopliae s. str. 也应包括 M. lepidiotae(异名),并且 M. anisopliae s. str. 通过其代谢组和较低的昆虫致病性与该复合体中的其他物种区分开来。提出了新的类群,即 M. hybridum、M. neoanisopliae 和 M. parapingshaense spp.这里提出的新类群有很强的系统发生组学支持,分生孢子/噬菌体长/宽的微尺度差异也证实了这一点,而代谢组学和毒力数据在很大程度上仍然是重叠的。我们还通过群体基因组学数据证明了局部克隆世系的存在,尤其是与已在田间应用三年的生物防治候选菌株的持续存在相对应的克隆世系。这项研究展示了将各种数据源结合起来,在一组重要的针对害虫的真菌生物控制剂中准确划分物种的实用性。
Integrative taxonomy of Metarhizium anisopliae species complex, based on phylogenomics combined with morphometrics, metabolomics, and virulence data
Metarhizium anisopliae (Clavicipitaceae, Hypocreales) is a globally distributed entomopathogenic fungus, which has been largely studied and used in agriculture for its potent entomopathogenicity. Since its taxonomic establishment as a member of Metarhizium, many closely related taxa have been described with highly similar morphology (cryptic species). A holotype specimen of M. anisopliae is not extant, and the ex-neotype strain (CBS 130.71) does not form a monophyletic clade with other strains, up to now, recognized as M. anisopliae sensu stricto. In this study, we have conducted an integrative taxonomic treatment of M. anisopliae sensu lato by including the ex-neotype strain of M. anisopliae, other unknown strains from our collections identified as M. anisopliae s. lat., as well as other known species that have been previously delimited as closely related but distinct to M. anisopliae. By including whole-genome sequencing, morphometric analysis, LC–MS based metabolomics, and virulence assays, we have demonstrated that M. anisopliae s. str. should also include M. lepidiotae (synonym), and that M. anisopliae s. str. differentiates from the other species of the complex by its metabolome and less severe entomopathogenicity. New taxa, namely M. hybridum, M. neoanisopliae and M. parapingshaense spp. nov., are proposed. The novel taxa proposed here have strong phylogenomics support, corroborated by fine-scale differences in the length/width of conidia/phialides, while the metabolomics and virulence data still largely overlap. We have also demonstrated via population genomics data the existence of local clonal lineages, particularly the one corresponding to the persistence of a biocontrol candidate strain that has been used in the field application for three years. This study showcases the utility of combining various data sources for accurate delimitation of species within an important group of fungal biocontrol agents against pest insects.
Ima FungusAgricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍:
The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue