Carlos Tamarit-Martínez, Lucía Bernat-Just, Carlos Bueno-López, Adrián M. Alambiaga-Caravaca, Virginia Merino, Alicia López-Castellano, Vicent Rodilla
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引用次数: 0
摘要
感染是关节置换术的主要并发症之一。这些感染很难治疗,因为导致感染的细菌会在假体中定居并形成生物膜,使抗菌剂无法到达感染区域。本研究是一个研究项目的一部分,该项目旨在开发能够加入抗菌剂的 3D 打印垫片(临时假体),用于关节成形术感染的个性化治疗。本研究的主要目的是分析基于聚乳酸(PLA)的三维打印结构层厚度对改善关节置换术感染治疗的影响。重点是以下参数:抗性、形态、药物释放以及打印临时假体中加入抗菌剂的效果。抗性研究表明,打印垫片的设计和层厚度对其抗性性能有影响。打印层的厚度会影响亚甲蓝(用作模型药物)的释放量。增加层厚度会导致更多的药物从间隔物中释放出来,这可能是孔隙率增加的结果。为了评估抗菌释放情况,在构建物中加入了氯唑西林和万古霉素。在三维构建体中加入氯唑西林和万古霉素后,这两种抗菌药物都得到了释放,金黄色葡萄球菌的生长抑制作用就证明了这一点。总之,初步结果表明,三维(3D)打印过程中间隔物的层厚度对药物释放起着重要作用。
An Antibacterial-Loaded PLA 3D-Printed Model for Temporary Prosthesis in Arthroplasty Infections: Evaluation of the Impact of Layer Thickness on the Mechanical Strength of a Construct and Drug Release
Infections are one of the main complications in arthroplasties. These infections are difficult to treat because the bacteria responsible for them settle in the prosthesis and form a biofilm that does not allow antimicrobials to reach the infected area. This study is part of a research project aimed at developing 3D-printed spacers (temporary prostheses) capable of incorporating antibacterials for the personalized treatment of arthroplasty infections. The main objective of this research was to analyze the impact of the layer thickness of 3D-printed constructs based on polylactic acid (PLA) for improved treatment of infections in arthroplasty. The focus is on the following parameters: resistance, morphology, drug release, and the effect of antibacterials incorporated in the printed temporary prostheses. The resistance studies revealed that the design and layer thickness of a printed spacer have an influence on its resistance properties. The thickness of the layer used in printing affects the amount of methylene blue (used as a model drug) that is released. Increasing layer thickness leads to a greater release of the drug from the spacer, probably as a result of higher porosity. To evaluate antibacterial release, cloxacillin and vancomycin were incorporated into the constructs. When incorporated into the 3D construct, both antibacterials were released, as evidenced by the growth inhibition of Staphylococcus aureus. In conclusion, preliminary results indicate that the layer thickness during the three-dimensional (3D) printing process of the spacer plays a significant role in drug release.
PharmaceuticsPharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍:
Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications, and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.