由 AlPcNE 介导的光动力疗法在小鼠黑色素瘤模型中引发的直接和缺席抗肿瘤反应

IF 4.9 3区 医学 Q1 PHARMACOLOGY & PHARMACY Pharmaceutics Pub Date : 2024-09-06 DOI:10.3390/pharmaceutics16091177
José Athayde Vasconcelos Morais, Pedro H. A. Barros, Marcelo de Macedo Brigido, Clara Luna Marina, Anamelia Bocca, André de Lima e Silva Mariano, Paulo E. N. de Souza, Karen L. R. Paiva, Marina Mesquita Simões, Sonia Nair Bao, Luana C. Camargo, João P. Figueiró Longo, Amanda Alencar Cabral Morais, Ricardo B. de Azevedo, Marcio J. P. Fonseca, Luis A. Muehlmann
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引用次数: 0

摘要

黑色素瘤是最具侵袭性的皮肤癌,由于其易转移和对传统疗法的顽固性,给临床带来了巨大挑战。尽管手术、化疗和放疗取得了进展,但晚期黑色素瘤的前景依然暗淡,因此迫切需要更有效的治疗方法。光动力疗法(PDT)已成为一种前景广阔的替代疗法,它能在对周围组织伤害最小的情况下靶向摧毁肿瘤。本研究评估了光动力疗法在双侧小鼠黑色素瘤模型中的直接和缺席抗肿瘤效果。虽然只治疗了两个肿瘤中的一个,但在两个肿瘤中都观察到了效果。我们的研究结果表明,血液分析和流式细胞术证明,治疗组的全身炎症发生了明显变化,CD4+和CD8+ T细胞群也发生了改变。高通量 RNA 测序(RNA-Seq)进一步揭示了治疗组和未治疗组肿瘤基因表达谱的变化。这项研究揭示了氯化铝酞菁纳米乳液(AlPcNE)介导的光动力疗法在黑色素瘤中的新型抗肿瘤和脱落效应,凸显了不同光动力疗法在调节免疫反应和实现更有效、更有针对性的癌症治疗方面的潜力。
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Direct and Abscopal Antitumor Responses Elicited by AlPcNE-Mediated Photodynamic Therapy in a Murine Melanoma Model
Melanoma, the most aggressive form of skin cancer, presents a major clinical challenge due to its tendency to metastasize and recalcitrance to traditional therapies. Despite advances in surgery, chemotherapy, and radiotherapy, the outlook for advanced melanoma remains bleak, reinforcing the urgent need for more effective treatments. Photodynamic therapy (PDT) has emerged as a promising alternative, leading to targeted tumor destruction with minimal harm to surrounding tissues. In this study, the direct and abscopal antitumor effects of PDT in a bilateral murine melanoma model were evaluated. Although only one of the two tumors was treated, effects were observed in both. Our findings revealed significant changes in systemic inflammation and alterations in CD4+ and CD8+ T cell populations in treated groups, as evidenced by blood analyses and flow cytometry. High-throughput RNA sequencing (RNA-Seq) further unveiled shifts in gene expression profiles in both treated and untreated tumors. This research sheds light on the novel antitumor and abscopal effects of nanoemulsion of aluminum chloride phthalocyanine (AlPcNE)-mediated PDT in melanoma, highlighting the potential of different PDT protocols to modulate immune responses and to achieve more effective and targeted cancer treatments.
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来源期刊
Pharmaceutics
Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
7.90
自引率
11.10%
发文量
2379
审稿时长
16.41 days
期刊介绍: Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications,  and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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