{"title":"利用遗传学和生物学方法管理大豆中的轮纹霉菌","authors":"Rafaela Bueno Loreto, Santino Aleandro da Silva, Andressa Cristina Zamboni Machado","doi":"10.1007/s40858-024-00687-9","DOIUrl":null,"url":null,"abstract":"<p><i>Rotylenchulus reniformis</i> poses a significant threat to cotton crops in the Brazilian Cerrado, particularly when grown consecutively with soybeans. This nematode has not only become a concern for cotton but has also led to considerable damage in soybean crops, emphasizing the need for effective nematode control in both agricultural settings. The aim of this study was to combine genetic control with the application of biological nematicides, as seed treatment, to manage <i>R. reniformis</i> under greenhouse conditions. Two soybean cultivars, TMG 4182 and Fibra, resistant and susceptible, were used and the biological nematicides used included <i>Purpureocillium lilacinum, Trichoderma harzianum + T. asperellum + Bacillus amyloliquefaciens</i>, <i>B. subtilis</i> + <i>B. licheniformis</i>, and <i>B. firmus</i>. Inoculation with 800 <i>R. reniformis</i> occurred in the cotyledonary stage, with evaluations conducted at 72 and 76 days after inoculation for Experiments 1 and 2, respectively. Nematodes were extracted from the soil and roots, calculating the reproduction factor (RF). The combination of biological nematicides with resistant cultivars did not yield substantial benefits in controlling reniform nematodes in soybean but safeguarding resistant cultivars through the application of chemical or biological nematicides is important to mitigate inoculum pressure on resistance genes. In addition, biological nematicides evaluated in this study did not improve soybean plant development and we concluded that managing reniform nematodes in soybean necessitates the integration of diverse control measures to effectively address the challenges posed by this nematode's impact on crops.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Management of Rotylenchulus reniformis in soybean using genetic and biological approaches\",\"authors\":\"Rafaela Bueno Loreto, Santino Aleandro da Silva, Andressa Cristina Zamboni Machado\",\"doi\":\"10.1007/s40858-024-00687-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><i>Rotylenchulus reniformis</i> poses a significant threat to cotton crops in the Brazilian Cerrado, particularly when grown consecutively with soybeans. 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引用次数: 0
摘要
根肿线虫(Rotylenchulus reniformis)对巴西塞拉多(Cerrado)地区的棉花作物构成严重威胁,尤其是与大豆连作时。这种线虫不仅是棉花的隐患,而且对大豆作物也造成了相当大的损害,因此在这两种农业环境中都需要对线虫进行有效控制。本研究的目的是在温室条件下,将基因控制与应用生物杀线虫剂作为种子处理相结合,以控制 R. reniformis。研究使用了两个大豆品种 TMG 4182 和 Fibra(抗性和易感性),使用的生物杀线虫剂包括 Purpureocillium lilacinum、Trichoderma harzianum + T. asperellum + Bacillus amyloliquefaciens、B. subtilis + B. licheniformis 和 B. firmus。800 R. reniformis 在子叶阶段接种,实验 1 和 2 分别在接种后 72 天和 76 天进行评估。从土壤和根部提取线虫,计算繁殖系数(RF)。生物杀线虫剂与抗性栽培品种的结合在控制大豆肾形线虫方面没有产生实质性的益处,但通过施用化学或生物杀线虫剂保护抗性栽培品种对减轻抗性基因的接种体压力非常重要。此外,本研究中评估的生物杀线虫剂并没有改善大豆植株的生长发育。我们的结论是,大豆肾形线虫的防治需要整合多种防治措施,以有效应对这种线虫对作物的影响所带来的挑战。
Management of Rotylenchulus reniformis in soybean using genetic and biological approaches
Rotylenchulus reniformis poses a significant threat to cotton crops in the Brazilian Cerrado, particularly when grown consecutively with soybeans. This nematode has not only become a concern for cotton but has also led to considerable damage in soybean crops, emphasizing the need for effective nematode control in both agricultural settings. The aim of this study was to combine genetic control with the application of biological nematicides, as seed treatment, to manage R. reniformis under greenhouse conditions. Two soybean cultivars, TMG 4182 and Fibra, resistant and susceptible, were used and the biological nematicides used included Purpureocillium lilacinum, Trichoderma harzianum + T. asperellum + Bacillus amyloliquefaciens, B. subtilis + B. licheniformis, and B. firmus. Inoculation with 800 R. reniformis occurred in the cotyledonary stage, with evaluations conducted at 72 and 76 days after inoculation for Experiments 1 and 2, respectively. Nematodes were extracted from the soil and roots, calculating the reproduction factor (RF). The combination of biological nematicides with resistant cultivars did not yield substantial benefits in controlling reniform nematodes in soybean but safeguarding resistant cultivars through the application of chemical or biological nematicides is important to mitigate inoculum pressure on resistance genes. In addition, biological nematicides evaluated in this study did not improve soybean plant development and we concluded that managing reniform nematodes in soybean necessitates the integration of diverse control measures to effectively address the challenges posed by this nematode's impact on crops.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.