生物测定指导下从藁本根提取物中分离驱虫剂化合物

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-09-08 DOI:10.1016/j.actatropica.2024.107386
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引用次数: 0

摘要

在我们针对具有医疗和兽医重要性的害虫筛选天然产品的计划中,Ligusticum porteri J.M.Coult. & Rose 根部的乙醇提取物对蚊子有显著的驱避作用。然后对提取物进行分馏,测试不同的馏分,以确定活性驱蚊化合物。测试的结果是分离出了不同的化合物,包括(Z)-3-亚丁基庚酞、(E)-3-亚丁基庚酞和(E/Z)-3-亚丁基庚酞的混合物。在 Klun 和 Debboun(K & D)的生物测定中,所有这些化合物对埃及伊蚊的叮咬威慑力与 N,N-二乙基-3-甲基苯甲酰胺(DEET)相似。(E/Z)-3-butylidenephthalide 是这两种化合物的混合物,在 Ali & Khan (A & K) 生物测定中进行了进一步测试。根据这些数据,该化合物的驱避性相似,而(E/Z)-3-亚丁烯基苯酞与胡萝卜素的混合物的 MED 值(6.25 + 6.25 = 12.5 µg/cm2)低于单个处理(25 µg/cm2)。在体内(皮肤直接涂抹生物测定),(E/Z)-3-丁烯基苯酞表现出卓越的驱避性。(E/Z)-3-亚丁烯基苯酞在施用率为 8% 和 16% 时的残留驱避时间分别为 4.5 小时和 10 小时,分别等于或优于 DEET 的 5 小时和 9 小时残留时间。(E/Z)-3-亚丁基酞酸酯与胡萝卜素的混合物(8 + 8 %)与单独施用 8 % 剂量的(E/Z)-3-亚丁基酞酸酯相比,残留驱避时间增加了 2 小时(44 %)。这些数据表明,(E/Z)-3-亚丁基酞菁是一种有效的驱蚊剂,性质稳定,保质期长。这种化合物的活性非同一般,残留时间与 DEET 相当。体内数据表明,(E/Z)-3-亚丁烯基苯酞作为一种驱蚊剂具有巨大的潜力,可以开发用于商业用途。不过,(E/Z)-3-亚丁基酞酸酯在 L. porteri 精油中的含量较低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Bioassay-guided isolation of insect repellent compounds from Ligusticum porteri root extract

In our program of screening natural products against the pests of medical and veterinary importance, ethanolic extract of the roots of Ligusticum porteri J.M.Coult. & Rose showed significant repellency against mosquitoes. The extract was then fractionated to test different fractions to identify the active repellent compounds. This testing resulted in the isolation of different compounds including (Z)-3-butylidenephthalide, (E)-3-butylidenephthalide, and a mixture of (E/Z)-3-butylidenephthalide. Biting deterrence of all these compounds was similar to N,N-diethyl-3-methylbenzamide (DEET) against Aedes aegypti (L.) in Klun and Debboun (K & D) bioassay. (E/Z)-3-butylidenephthalide which is a mixture of the two compounds was further tested in Ali & Khan (A & K) bioassay. Based on these data repellency of this compound was similar whereas the MED values of the mixtures of (E/Z)-3-butylidenephthalide with carotol were lower (6.25 + 6.25 = 12.5 µg/cm2) than individual treatments (25 µg/cm2). In in vivo (direct skin application bioassay), (E/Z)-3-butylidenephthalide showed excellent repellency. The residual repellency of (E/Z)-3-butylidenephthalide at 8 and 16 % application rates was 4.5 and 10-h respectively which was equal to or better than DEET with the residual time of 5 and 9-h, respectively. The mixture of (E/Z)-3-butylidenephthalide with carotol (8 + 8 %) increased the residual repellency by 2-h (44 %) as compared to (E/Z)-3-butylidenephthalide alone at a dose of 8 %. These data indicated that (E/Z)-3-butylidenephthalide is an effective mosquito repellent that is stable and has a long shelf life. The activity of this compound is extraordinary and residual time is comparable to DEET. In vivo data demonstrated an enormous potential of (E/Z)-3-butylidenephthalide as a repellent that can be developed for commercial use. However, (E/Z)-3-butylidenephthalate was found in lower amounts of the L. porteri essential oil.

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来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
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