Ulli Rothweiler, Sigurd Eidem Gundesø, Emma Wu Mikalsen, Steingrim Svenning, Mahavir Singh, Francis Combes, Frida J Pettersson, Antonia Mangold, Yvonne Piotrowski, Felix Schwab, Olav Lanes, Bernd Ketelsen Striberny
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引用次数: 0
摘要
过去五十年来,DNA 限制酶彻底改变了生物技术。这些酶被广泛应用于 DNA 研究和 DNA 工程,而新兴的 RNA 和 mRNA 治疗领域则需要序列特异的 RNA 内切核酸酶。在这里,我们介绍了序列特异性 RNA 内切核酸酶 III 型毒素-抗毒素家族的成员 EcoToxN1 及其在 RNA 和 mRNA 分析中的应用。这种酶能识别单链 RNA 中的特定五聚体,并在此序列内切割 RNA。这种酶既不依赖于引导 RNA 或 DNA 寡聚物与模板的退火,也不需要镁。此外,它的工作温度范围很广。凭借其独特的功能和特性,EcoToxN1 可被归类为一种 RNA 限制酶。EcoToxN1 为 RNA 分析和生物制造带来了新的工作流程,满足了对更快、更便宜、更可靠的分析方法的需求。
Using nucleolytic toxins as restriction enzymes enables new RNA applications
Over the past five decades, DNA restriction enzymes have revolutionized biotechnology. While these enzymes are widely used in DNA research and DNA engineering, the emerging field of RNA and mRNA therapeutics requires sequence-specific RNA endoribonucleases. Here, we describe EcoToxN1, a member of the type III toxin-antitoxin family of sequence-specific RNA endoribonucleases, and its use in RNA and mRNA analysis. This enzyme recognizes a specific pentamer in a single-stranded RNA and cleaves the RNA within this sequence. The enzyme is neither dependent on annealing of guide RNA or DNA oligos to the template nor does it require magnesium. Furthermore, it performs over a wide range of temperatures. With its unique functions and characteristics, EcoToxN1 can be classified as an RNA restriction enzyme. EcoToxN1 enables new workflows in RNA analysis and biomanufacturing, meeting the demand for faster, cheaper, and more robust analysis methods.
期刊介绍:
Nucleic Acids Research (NAR) is a scientific journal that publishes research on various aspects of nucleic acids and proteins involved in nucleic acid metabolism and interactions. It covers areas such as chemistry and synthetic biology, computational biology, gene regulation, chromatin and epigenetics, genome integrity, repair and replication, genomics, molecular biology, nucleic acid enzymes, RNA, and structural biology. The journal also includes a Survey and Summary section for brief reviews. Additionally, each year, the first issue is dedicated to biological databases, and an issue in July focuses on web-based software resources for the biological community. Nucleic Acids Research is indexed by several services including Abstracts on Hygiene and Communicable Diseases, Animal Breeding Abstracts, Agricultural Engineering Abstracts, Agbiotech News and Information, BIOSIS Previews, CAB Abstracts, and EMBASE.