{"title":"肠道微生物组的变化可能与癫痫患者突然发作加重有关","authors":"","doi":"10.1016/j.clineuro.2024.108556","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Seizures can be triggered by a variety of endogenous or exogenous factors. We hypothesized that alterations in the gut microbiome may be a seizure precipitant and analyzed the composition and characteristics of the gut microbiome in epilepsy patients who experienced an abrupt seizure exacerbation without a clear seizure precipitant.</p></div><div><h3>Methods</h3><p>We prospectively enrolled 25 adult patients with epilepsy and collected fecal samples on the admission and after seizure recovery for next-generation sequencing analysis. We performed nonparametric paired t-test analysis to evaluate changes in the gut microbiota as seizures worsened and when it recovered and also estimated alpha and beta diversities in each category.</p></div><div><h3>Results</h3><p>A total of 19 patients (13 males) aged between 19 and 78 years (mean 45.2 years) were included in the study. The composition of the gut microbiota underwent a significant change following an abrupt seizure exacerbation. At the phylum level, the relative abundance of <em>Fusobacteria</em> and <em>Synergistetes</em> was decreased in the seizure recovery state compared to the acute seizure exacerbation. A similar trend was observed at the lower hierarchical levels, with a decrease in the relative abundance of <em>Fusobacteria</em>, <em>Tissierellia</em>, and <em>Synergistia</em> at the class level, and that of <em>Synergistales</em>, <em>Tissierellales</em>, and <em>Fusobacteriales</em> at the order level. At the family level, the relative abundance of <em>Fusobacteriaceae</em> and <em>Staphylococcaceae</em> was decreased, whereas that of <em>Leuconostocaceae</em> was increased. No statistical differences were observed in alpha and beta diversity between the pre- and post-acute seizure exacerbation periods.</p></div><div><h3>Significance</h3><p>Our study suggests that the changes in <em>Fusobacteriaceae</em> and <em>Lecuonostocaceae</em> may be associated with acute seizure exacerbation in epilepsy patients. Given that <em>Fusobacteriaceae</em> are associated with various systemic diseases due to their invasive properties and that <em>Leuconostocaceae</em> are known to produce GABA, our results may suggest a gut microbiome-based treatment option for epilepsy patients.</p></div>","PeriodicalId":10385,"journal":{"name":"Clinical Neurology and Neurosurgery","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Changes in gut microbiome can be associated with abrupt seizure exacerbation in epilepsy patients\",\"authors\":\"\",\"doi\":\"10.1016/j.clineuro.2024.108556\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Seizures can be triggered by a variety of endogenous or exogenous factors. We hypothesized that alterations in the gut microbiome may be a seizure precipitant and analyzed the composition and characteristics of the gut microbiome in epilepsy patients who experienced an abrupt seizure exacerbation without a clear seizure precipitant.</p></div><div><h3>Methods</h3><p>We prospectively enrolled 25 adult patients with epilepsy and collected fecal samples on the admission and after seizure recovery for next-generation sequencing analysis. We performed nonparametric paired t-test analysis to evaluate changes in the gut microbiota as seizures worsened and when it recovered and also estimated alpha and beta diversities in each category.</p></div><div><h3>Results</h3><p>A total of 19 patients (13 males) aged between 19 and 78 years (mean 45.2 years) were included in the study. The composition of the gut microbiota underwent a significant change following an abrupt seizure exacerbation. At the phylum level, the relative abundance of <em>Fusobacteria</em> and <em>Synergistetes</em> was decreased in the seizure recovery state compared to the acute seizure exacerbation. A similar trend was observed at the lower hierarchical levels, with a decrease in the relative abundance of <em>Fusobacteria</em>, <em>Tissierellia</em>, and <em>Synergistia</em> at the class level, and that of <em>Synergistales</em>, <em>Tissierellales</em>, and <em>Fusobacteriales</em> at the order level. At the family level, the relative abundance of <em>Fusobacteriaceae</em> and <em>Staphylococcaceae</em> was decreased, whereas that of <em>Leuconostocaceae</em> was increased. No statistical differences were observed in alpha and beta diversity between the pre- and post-acute seizure exacerbation periods.</p></div><div><h3>Significance</h3><p>Our study suggests that the changes in <em>Fusobacteriaceae</em> and <em>Lecuonostocaceae</em> may be associated with acute seizure exacerbation in epilepsy patients. Given that <em>Fusobacteriaceae</em> are associated with various systemic diseases due to their invasive properties and that <em>Leuconostocaceae</em> are known to produce GABA, our results may suggest a gut microbiome-based treatment option for epilepsy patients.</p></div>\",\"PeriodicalId\":10385,\"journal\":{\"name\":\"Clinical Neurology and Neurosurgery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Neurology and Neurosurgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0303846724004438\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CLINICAL NEUROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Neurology and Neurosurgery","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0303846724004438","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}
Changes in gut microbiome can be associated with abrupt seizure exacerbation in epilepsy patients
Objective
Seizures can be triggered by a variety of endogenous or exogenous factors. We hypothesized that alterations in the gut microbiome may be a seizure precipitant and analyzed the composition and characteristics of the gut microbiome in epilepsy patients who experienced an abrupt seizure exacerbation without a clear seizure precipitant.
Methods
We prospectively enrolled 25 adult patients with epilepsy and collected fecal samples on the admission and after seizure recovery for next-generation sequencing analysis. We performed nonparametric paired t-test analysis to evaluate changes in the gut microbiota as seizures worsened and when it recovered and also estimated alpha and beta diversities in each category.
Results
A total of 19 patients (13 males) aged between 19 and 78 years (mean 45.2 years) were included in the study. The composition of the gut microbiota underwent a significant change following an abrupt seizure exacerbation. At the phylum level, the relative abundance of Fusobacteria and Synergistetes was decreased in the seizure recovery state compared to the acute seizure exacerbation. A similar trend was observed at the lower hierarchical levels, with a decrease in the relative abundance of Fusobacteria, Tissierellia, and Synergistia at the class level, and that of Synergistales, Tissierellales, and Fusobacteriales at the order level. At the family level, the relative abundance of Fusobacteriaceae and Staphylococcaceae was decreased, whereas that of Leuconostocaceae was increased. No statistical differences were observed in alpha and beta diversity between the pre- and post-acute seizure exacerbation periods.
Significance
Our study suggests that the changes in Fusobacteriaceae and Lecuonostocaceae may be associated with acute seizure exacerbation in epilepsy patients. Given that Fusobacteriaceae are associated with various systemic diseases due to their invasive properties and that Leuconostocaceae are known to produce GABA, our results may suggest a gut microbiome-based treatment option for epilepsy patients.
期刊介绍:
Clinical Neurology and Neurosurgery is devoted to publishing papers and reports on the clinical aspects of neurology and neurosurgery. It is an international forum for papers of high scientific standard that are of interest to Neurologists and Neurosurgeons world-wide.