{"title":"模拟小鼠视网膜血液动力学对尾部悬浮的反应","authors":"","doi":"10.1016/j.compbiomed.2024.109148","DOIUrl":null,"url":null,"abstract":"<div><p>The etiology of spaceflight-associated neuro-ocular syndrome (SANS) remains unclear. Recent murine studies indicate there may be a link between the space environment and retinal endothelial dysfunction.</p><p>Post-fixed control (N = 4) and 14-day tail-suspended (TS) (N = 4) mice eye samples were stained and imaged for the vessel plexus and co-located regions of endothelial cell death. A custom workflow combined whole-mounted and tear reconstructed three-dimensional (3D) spherical retinal plexus models with computational fluid dynamics (CFD) simulation that accounted for the Fåhræus-Lindqvist effect and boundary conditions that accommodated TS fluid pressure measurements and deeper capillary layer blood flow distribution.</p><p>TS samples exhibited reduced surface area (4.6 ± 0.5 mm<sup>2</sup> vs. 3.5 ± 0.3 mm<sup>2</sup>, P = 0.010) and shorter lengths between branches in small vessels (<10 μm, 69.5 ± 0.6 μm vs. 60.4 ± 1.1 μm, P < 0.001). Wall shear stress (WSS) and pressure were higher in TS mice compared to controls, particularly in smaller vessels (<10 μm, WSS: 6.57 ± 1.08 Pa vs. 4.72 ± 0.67 Pa, P = 0.034, Pressure: 72.04 ± 3.14 mmHg vs. 50.64 ± 6.74 mmHg, P = 0.004). Rates of retinal endothelial cell death were variable in TS mice compared to controls. WSS and pressure were generally higher in cell death regions, both within and between cohorts, but significance was variable and limited to small to medium-sized vessels (<20 μm).</p><p>These findings suggest a link may exist between emulated microgravity and retinal endothelial dysfunction that may have implications for SANS development. 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引用次数: 0
摘要
太空飞行相关神经眼综合征(SANS)的病因尚不清楚。对固定后的对照组(4 只)和 14 天的尾悬浮(TS)组(4 只)小鼠眼球样本进行染色和成像,以观察血管丛和内皮细胞死亡的共定位区域。定制的工作流程将整体安装和撕裂重建的三维(3D)球形视网膜血管丛模型与计算流体动力学(CFD)模拟结合在一起,计算流体动力学模拟考虑了Fåhræus-Lindqvist效应和边界条件,以适应TS流体压力测量和更深的毛细血管层血流分布。TS 样品的表面积缩小(4.6 ± 0.5 mm2 vs. 3.5 ± 0.3 mm2,P = 0.010),小血管分支间的长度缩短(10 μm,69.5 ± 0.6 μm vs. 60.4 ± 1.1 μm,P <0.001)。与对照组相比,TS 小鼠的血管壁剪切应力(WSS)和压力较高,尤其是在较小的血管中(<10 μm, WSS: 6.57 ± 1.08 Pa vs. 4.72 ± 0.67 Pa, P = 0.034, Pressure: 72.04 ± 3.14 mmHg vs. 50.64 ± 6.74 mmHg, P = 0.004)。与对照组相比,TS 小鼠视网膜内皮细胞的死亡率各不相同。这些发现表明,模拟微重力与视网膜内皮功能障碍之间可能存在联系,这可能对SANS的发展有影响。今后的工作应考虑增加更大样本量的物种或太空飞行队列。
Simulation of murine retinal hemodynamics in response to tail suspension
The etiology of spaceflight-associated neuro-ocular syndrome (SANS) remains unclear. Recent murine studies indicate there may be a link between the space environment and retinal endothelial dysfunction.
Post-fixed control (N = 4) and 14-day tail-suspended (TS) (N = 4) mice eye samples were stained and imaged for the vessel plexus and co-located regions of endothelial cell death. A custom workflow combined whole-mounted and tear reconstructed three-dimensional (3D) spherical retinal plexus models with computational fluid dynamics (CFD) simulation that accounted for the Fåhræus-Lindqvist effect and boundary conditions that accommodated TS fluid pressure measurements and deeper capillary layer blood flow distribution.
TS samples exhibited reduced surface area (4.6 ± 0.5 mm2 vs. 3.5 ± 0.3 mm2, P = 0.010) and shorter lengths between branches in small vessels (<10 μm, 69.5 ± 0.6 μm vs. 60.4 ± 1.1 μm, P < 0.001). Wall shear stress (WSS) and pressure were higher in TS mice compared to controls, particularly in smaller vessels (<10 μm, WSS: 6.57 ± 1.08 Pa vs. 4.72 ± 0.67 Pa, P = 0.034, Pressure: 72.04 ± 3.14 mmHg vs. 50.64 ± 6.74 mmHg, P = 0.004). Rates of retinal endothelial cell death were variable in TS mice compared to controls. WSS and pressure were generally higher in cell death regions, both within and between cohorts, but significance was variable and limited to small to medium-sized vessels (<20 μm).
These findings suggest a link may exist between emulated microgravity and retinal endothelial dysfunction that may have implications for SANS development. Future work with increased sample sizes of larger species or spaceflight cohorts should be considered.
期刊介绍:
Computers in Biology and Medicine is an international forum for sharing groundbreaking advancements in the use of computers in bioscience and medicine. This journal serves as a medium for communicating essential research, instruction, ideas, and information regarding the rapidly evolving field of computer applications in these domains. By encouraging the exchange of knowledge, we aim to facilitate progress and innovation in the utilization of computers in biology and medicine.