Yuexun Tian , Jose G. Juarez , Andrea M. Moller-Vasquez , María Granados-Presa , Francisco C. Ferreira , Pamela M. Pennington , Norma Padilla , Gabriel L. Hamer , Sarah A. Hamer
{"title":"危地马拉农村地区作为致病立克次体和巴顿氏菌哨兵的狗体外寄生虫","authors":"Yuexun Tian , Jose G. Juarez , Andrea M. Moller-Vasquez , María Granados-Presa , Francisco C. Ferreira , Pamela M. Pennington , Norma Padilla , Gabriel L. Hamer , Sarah A. Hamer","doi":"10.1016/j.actatropica.2024.107401","DOIUrl":null,"url":null,"abstract":"<div><p>Fleas and ticks serve as vectors of multiple pathogens in the genera <em>Rickettsia</em> and <em>Bartonella</em> that cause diseases in humans and other animals. Although human rickettsiosis and bartonellosis have been reported in all countries in Central America, limited research has been conducted to investigate the natural cycles of flea- and tick-borne rickettsiosis and bartonellosis, especially in Guatemala. We evaluated dog parasites as sentinels for zoonotic disease risk in rural Guatemala by sampling ticks and fleas from dogs, which were then identified and individually screened for <em>Rickettsia</em> and <em>Bartonella</em>. A total of 77 households were surveyed and 80.5 % of them had dogs. Overall, 133 dogs were examined for fleas and ticks, of which 68.4 % had fleas and 35.3 % had ticks. A total of 433 fleas and 181 ticks were collected from the infested dogs, with an additional 33 ticks collected from house walls. Three flea species were identified: <em>Ctenocephalides felis</em> (70.0 %), <em>Echidnophaga gallinacea</em> (11.8 %), and <em>Pulex</em> sp. (17.8 %). Among the collected ticks, 97 % were identified as <em>Rhipicephalus sanguineus</em> sensu lato with the rest being <em>Amblyomma cajennense, A. auricularium</em>, and <em>A. ovale. Rickettsia felis</em> were detected in six <em>C. felis</em>, in one <em>Pulex</em> sp., and in two <em>R. sanguineus</em> sensu lato, while <em>Candidatus</em> R. senegalensis was detected in one <em>C. felis. Bartonella</em> was detected only in fleas, including three <em>Pulex</em> sp. infected with <em>B. vinsonii</em> subsp. <em>berkhoffii, B. henselae</em>, and <em>Bartonella</em> sp., respectively, and 11 <em>C. felis</em> infected with <em>B. henselae</em>. This study reports <em>Candidatus</em> R. senegalensis and <em>B. vinsonii</em> subsp. <em>berkhoffii</em> in Guatemala for the first time, and indicates the potential risk of human and dog exposure to <em>Rickettsia</em> and <em>Bartonella</em> species. These results show that dogs provide critical information relevant to managing human potential exposure to flea- and tick-borne pathogens in rural Guatemala. This approach can potentially be expanded to other regions in Central America where domestic dogs are abundant and suffer from ectoparasite infestation.</p></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"260 ","pages":"Article 107401"},"PeriodicalIF":2.1000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0001706X24002821/pdfft?md5=79dbb534e8483daee786fd4c6a96eeda&pid=1-s2.0-S0001706X24002821-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Dog ectoparasites as sentinels for pathogenic Rickettsia and Bartonella in rural Guatemala\",\"authors\":\"Yuexun Tian , Jose G. Juarez , Andrea M. Moller-Vasquez , María Granados-Presa , Francisco C. Ferreira , Pamela M. Pennington , Norma Padilla , Gabriel L. Hamer , Sarah A. Hamer\",\"doi\":\"10.1016/j.actatropica.2024.107401\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Fleas and ticks serve as vectors of multiple pathogens in the genera <em>Rickettsia</em> and <em>Bartonella</em> that cause diseases in humans and other animals. Although human rickettsiosis and bartonellosis have been reported in all countries in Central America, limited research has been conducted to investigate the natural cycles of flea- and tick-borne rickettsiosis and bartonellosis, especially in Guatemala. We evaluated dog parasites as sentinels for zoonotic disease risk in rural Guatemala by sampling ticks and fleas from dogs, which were then identified and individually screened for <em>Rickettsia</em> and <em>Bartonella</em>. A total of 77 households were surveyed and 80.5 % of them had dogs. Overall, 133 dogs were examined for fleas and ticks, of which 68.4 % had fleas and 35.3 % had ticks. A total of 433 fleas and 181 ticks were collected from the infested dogs, with an additional 33 ticks collected from house walls. Three flea species were identified: <em>Ctenocephalides felis</em> (70.0 %), <em>Echidnophaga gallinacea</em> (11.8 %), and <em>Pulex</em> sp. (17.8 %). Among the collected ticks, 97 % were identified as <em>Rhipicephalus sanguineus</em> sensu lato with the rest being <em>Amblyomma cajennense, A. auricularium</em>, and <em>A. ovale. Rickettsia felis</em> were detected in six <em>C. felis</em>, in one <em>Pulex</em> sp., and in two <em>R. sanguineus</em> sensu lato, while <em>Candidatus</em> R. senegalensis was detected in one <em>C. felis. Bartonella</em> was detected only in fleas, including three <em>Pulex</em> sp. infected with <em>B. vinsonii</em> subsp. <em>berkhoffii, B. henselae</em>, and <em>Bartonella</em> sp., respectively, and 11 <em>C. felis</em> infected with <em>B. henselae</em>. This study reports <em>Candidatus</em> R. senegalensis and <em>B. vinsonii</em> subsp. <em>berkhoffii</em> in Guatemala for the first time, and indicates the potential risk of human and dog exposure to <em>Rickettsia</em> and <em>Bartonella</em> species. These results show that dogs provide critical information relevant to managing human potential exposure to flea- and tick-borne pathogens in rural Guatemala. This approach can potentially be expanded to other regions in Central America where domestic dogs are abundant and suffer from ectoparasite infestation.</p></div>\",\"PeriodicalId\":7240,\"journal\":{\"name\":\"Acta tropica\",\"volume\":\"260 \",\"pages\":\"Article 107401\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0001706X24002821/pdfft?md5=79dbb534e8483daee786fd4c6a96eeda&pid=1-s2.0-S0001706X24002821-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta tropica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0001706X24002821\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0001706X24002821","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Dog ectoparasites as sentinels for pathogenic Rickettsia and Bartonella in rural Guatemala
Fleas and ticks serve as vectors of multiple pathogens in the genera Rickettsia and Bartonella that cause diseases in humans and other animals. Although human rickettsiosis and bartonellosis have been reported in all countries in Central America, limited research has been conducted to investigate the natural cycles of flea- and tick-borne rickettsiosis and bartonellosis, especially in Guatemala. We evaluated dog parasites as sentinels for zoonotic disease risk in rural Guatemala by sampling ticks and fleas from dogs, which were then identified and individually screened for Rickettsia and Bartonella. A total of 77 households were surveyed and 80.5 % of them had dogs. Overall, 133 dogs were examined for fleas and ticks, of which 68.4 % had fleas and 35.3 % had ticks. A total of 433 fleas and 181 ticks were collected from the infested dogs, with an additional 33 ticks collected from house walls. Three flea species were identified: Ctenocephalides felis (70.0 %), Echidnophaga gallinacea (11.8 %), and Pulex sp. (17.8 %). Among the collected ticks, 97 % were identified as Rhipicephalus sanguineus sensu lato with the rest being Amblyomma cajennense, A. auricularium, and A. ovale. Rickettsia felis were detected in six C. felis, in one Pulex sp., and in two R. sanguineus sensu lato, while Candidatus R. senegalensis was detected in one C. felis. Bartonella was detected only in fleas, including three Pulex sp. infected with B. vinsonii subsp. berkhoffii, B. henselae, and Bartonella sp., respectively, and 11 C. felis infected with B. henselae. This study reports Candidatus R. senegalensis and B. vinsonii subsp. berkhoffii in Guatemala for the first time, and indicates the potential risk of human and dog exposure to Rickettsia and Bartonella species. These results show that dogs provide critical information relevant to managing human potential exposure to flea- and tick-borne pathogens in rural Guatemala. This approach can potentially be expanded to other regions in Central America where domestic dogs are abundant and suffer from ectoparasite infestation.
期刊介绍:
Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.