Olinda Briski, Juan P. Cabeza, Daniel F. Salamone, Rafael Fernández-Martin, Andrés Gambini
{"title":"锌螯合剂 1,10-菲罗啉在猪卵胞浆内单精子显微注射后辅助激活卵母细胞的效率","authors":"Olinda Briski, Juan P. Cabeza, Daniel F. Salamone, Rafael Fernández-Martin, Andrés Gambini","doi":"10.1071/rd24129","DOIUrl":null,"url":null,"abstract":"<strong> Context</strong><p><i>In vitro</i> embryo production in pigs is an important tool for advancing biomedical research. Intracytoplasmic sperm injection (ICSI) circumvents the polyspermy problems associated with conventional IVF in porcine. However, the suboptimal efficiency for ICSI in pigs requires new strategies to increase blastocyst formation rates.</p><strong> Aim</strong><p>To investigate novel methods for assisted activation using the zinc chelator 1,10-phenanthroline (PHEN), and to improve embryo developmental competence and quality of ICSI porcine blastocyst.</p><strong> Methods</strong><p>ICSI embryos were treated with PHEN after or before sperm injection, recording pronuclear formation, blastocyst rate and the expression of SMARCA4, OCT4, SOX2 and CDX2.</p><strong> Key results</strong><p>Neither electrical nor PHEN significantly improves pronuclear formation rates before or after ICSI. Following <i>in vitro</i> culture to the blastocyst stage, no significant differences were observed in developmental rates among the groups. Moreover, the use of PHEN did not alter the total cell number or the expression of OCT4, SOX2 and CDX2 in pig ICSI blastocysts.</p><strong> Conclusions</strong><p>Assisted oocyte activation with PHEN does not affect the preimplantation development of ICSI-derived pig embryos.</p><strong> Implications</strong><p>These results hold significance in refining and advancing the application of assisted oocyte activation techniques. They offer insights into addressing fertility issues and propelling advancements in human and animal reproductive medicine.</p>","PeriodicalId":20932,"journal":{"name":"Reproduction, fertility, and development","volume":"31 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Efficiency of the zinc chelator 1,10-phenanthroline for assisted oocyte activation following ICSI in pigs\",\"authors\":\"Olinda Briski, Juan P. Cabeza, Daniel F. Salamone, Rafael Fernández-Martin, Andrés Gambini\",\"doi\":\"10.1071/rd24129\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<strong> Context</strong><p><i>In vitro</i> embryo production in pigs is an important tool for advancing biomedical research. Intracytoplasmic sperm injection (ICSI) circumvents the polyspermy problems associated with conventional IVF in porcine. However, the suboptimal efficiency for ICSI in pigs requires new strategies to increase blastocyst formation rates.</p><strong> Aim</strong><p>To investigate novel methods for assisted activation using the zinc chelator 1,10-phenanthroline (PHEN), and to improve embryo developmental competence and quality of ICSI porcine blastocyst.</p><strong> Methods</strong><p>ICSI embryos were treated with PHEN after or before sperm injection, recording pronuclear formation, blastocyst rate and the expression of SMARCA4, OCT4, SOX2 and CDX2.</p><strong> Key results</strong><p>Neither electrical nor PHEN significantly improves pronuclear formation rates before or after ICSI. Following <i>in vitro</i> culture to the blastocyst stage, no significant differences were observed in developmental rates among the groups. Moreover, the use of PHEN did not alter the total cell number or the expression of OCT4, SOX2 and CDX2 in pig ICSI blastocysts.</p><strong> Conclusions</strong><p>Assisted oocyte activation with PHEN does not affect the preimplantation development of ICSI-derived pig embryos.</p><strong> Implications</strong><p>These results hold significance in refining and advancing the application of assisted oocyte activation techniques. They offer insights into addressing fertility issues and propelling advancements in human and animal reproductive medicine.</p>\",\"PeriodicalId\":20932,\"journal\":{\"name\":\"Reproduction, fertility, and development\",\"volume\":\"31 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reproduction, fertility, and development\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1071/rd24129\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduction, fertility, and development","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1071/rd24129","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Efficiency of the zinc chelator 1,10-phenanthroline for assisted oocyte activation following ICSI in pigs
Context
In vitro embryo production in pigs is an important tool for advancing biomedical research. Intracytoplasmic sperm injection (ICSI) circumvents the polyspermy problems associated with conventional IVF in porcine. However, the suboptimal efficiency for ICSI in pigs requires new strategies to increase blastocyst formation rates.
Aim
To investigate novel methods for assisted activation using the zinc chelator 1,10-phenanthroline (PHEN), and to improve embryo developmental competence and quality of ICSI porcine blastocyst.
Methods
ICSI embryos were treated with PHEN after or before sperm injection, recording pronuclear formation, blastocyst rate and the expression of SMARCA4, OCT4, SOX2 and CDX2.
Key results
Neither electrical nor PHEN significantly improves pronuclear formation rates before or after ICSI. Following in vitro culture to the blastocyst stage, no significant differences were observed in developmental rates among the groups. Moreover, the use of PHEN did not alter the total cell number or the expression of OCT4, SOX2 and CDX2 in pig ICSI blastocysts.
Conclusions
Assisted oocyte activation with PHEN does not affect the preimplantation development of ICSI-derived pig embryos.
Implications
These results hold significance in refining and advancing the application of assisted oocyte activation techniques. They offer insights into addressing fertility issues and propelling advancements in human and animal reproductive medicine.
期刊介绍：
Reproduction, Fertility and Development is an international journal for the publication of original and significant contributions on vertebrate reproductive and developmental biology. Subject areas include, but are not limited to: physiology, biochemistry, cell and molecular biology, endocrinology, genetics and epigenetics, behaviour, immunology and the development of reproductive technologies in humans, livestock and wildlife, and in pest management.
Reproduction, Fertility and Development is a valuable resource for research scientists working in industry or academia on reproductive and developmental biology, clinicians and veterinarians interested in the basic science underlying their disciplines, and students.
Reproduction, Fertility and Development is the official journal of the International Embryo Technology Society and the Society for Reproductive Biology.
Reproduction, Fertility and Development is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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