用于软骨细胞培养的琼脂糖-胶原复合水凝胶的表征

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL Annals of Biomedical Engineering Pub Date : 2024-09-14 DOI:10.1007/s10439-024-03613-x
Clarisse Zigan, Claudia Benito Alston, Aritra Chatterjee, Luis Solorio, Deva D. Chan
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引用次数: 0

摘要

要阐明细胞的机械传导机制,就必须采用能有效融合生物功能与适当机械特性的生物材料。琼脂糖和胶原蛋白分别是软骨机械生物学和机械传导研究中常用的生物聚合物,但它们缺乏使其成为功能工程软骨理想材料的特性。在这项研究中,琼脂糖与 I 型胶原混合,制成最终浓度为 4% w/v 或 2% w/v 的琼脂糖与 2 mg/mL 胶原的水凝胶。我们假设,在高浓度琼脂糖水凝胶中加入胶原蛋白不会降低机械性能。除了细胞增殖和硫酸化氨基糖的生成外,我们还完成了细胞和细胞负载研究,以评估流变和压缩特性、收缩和结构均匀性。在 21 天的培养过程中,接种了原代小鼠软骨细胞的 4% 琼脂糖-2 毫克/毫升胶原 I 水凝胶显示出的结构和体积机械行为与纯 4% 琼脂糖水凝胶、细胞增殖和持续糖胺聚糖生成相比没有显著变化,这表明有望开发出一种有效的水凝胶,用于软骨细胞机械传导和机械生物学研究。
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Characterization of Composite Agarose–Collagen Hydrogels for Chondrocyte Culture

To elucidate the mechanisms of cellular mechanotransduction, it is necessary to employ biomaterials that effectively merge biofunctionality with appropriate mechanical characteristics. Agarose and collagen separately are common biopolymers used in cartilage mechanobiology and mechanotransduction studies but lack features that make them ideal for functional engineered cartilage. In this study, agarose is blended with collagen type I to create hydrogels with final concentrations of 4% w/v or 2% w/v agarose with 2 mg/mL collagen. We hypothesized that the addition of collagen into a high-concentration agarose hydrogel does not diminish mechanical properties. Acellular and cell-laden studies were completed to assess rheologic and compressive properties, contraction, and structural homogeneity in addition to cell proliferation and sulfated glycosaminoglycan production. Over 21 days in culture, cellular 4% agarose–2 mg/mL collagen I hydrogels seeded with primary murine chondrocytes displayed structural and bulk mechanical behaviors that did not significantly alter from 4% agarose-only hydrogels, cell proliferation, and continual glycosaminoglycan production, indicating promise toward the development of an effective hydrogel for chondrocyte mechanotransduction and mechanobiology studies.

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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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