G. S. Amithabh, M. P. Gireesh Kumar, Kaviya Selvapaniyan, Baskar Baburaj
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引用次数: 0
摘要
维生素 D 及其代谢物分析方法的最新进展大大提高了我们在生物液体、真菌、天然食品和强化食品等多种来源中精确测定和量化这些物质的能力。本研究重点关注样品制备程序的最新进展,包括固相萃取和环境友好型萃取方法。这些方法旨在提高效率并尽量减少溶剂的使用。此外,我们还探讨了日益流行的色谱法,特别是 LC-MS/MS 和正在开发的超临界流体色谱法 (SFC),这些方法提高了灵敏度和选择性,并缩短了维生素 D 综合分析的分析时间。这些发展对于克服维生素 D 研究中低浓度和复杂基质带来的挑战至关重要。
Recent Development on the Extraction, Detection, and Quantification of Vitamin D from Various Sources – An Update
Recent advancements in analytical methods for vitamin D and its metabolites have substantially enhanced our capacity to precisely determine and quantify these substances in a wide range of sources, such as biological fluids, fungus, natural and fortified foods. This study focuses on the latest advancements in sample preparation procedures, including solid-phase extraction and environmentally friendly extraction methods. These approaches aim to enhance efficiency and minimize the use of solvents. In addition, we explore the growing popularity of chromatographic methods, specifically LC-MS/MS and developing supercritical fluid chromatography (SFC), which provide improved sensitivity, selectivity, and faster analytical times for comprehensive vitamin D profiling. These developments are crucial for overcoming the challenges presented by low concentrations and complex matrices in the investigation of vitamin D.