Wei Du, Mingxuan Zhou, Guancheng Wang, Mingze Lu, Xiao Wang, Zhirui Guo, Ming Ma, Yu Zhang
{"title":"基于 HPRR 的二原子催化剂电化学生物传感器用于检测与癌症有关的细胞外囊泡","authors":"Wei Du, Mingxuan Zhou, Guancheng Wang, Mingze Lu, Xiao Wang, Zhirui Guo, Ming Ma, Yu Zhang","doi":"10.1039/d4ay01573h","DOIUrl":null,"url":null,"abstract":"Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis due to their rich content of tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR) based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst catalytic HPRR current amplification strategies for specific identification and highly sensitive detection. Anti-CXCR4 antibody was immobilized on Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bounded to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 107 particles mL-1, with a detection limit of 117 particles mL-1, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"HPRR-Based Diatomic Catalyst Electrochemistry Biosensor for Detecting Cancer-Related Extracellular Vesicles\",\"authors\":\"Wei Du, Mingxuan Zhou, Guancheng Wang, Mingze Lu, Xiao Wang, Zhirui Guo, Ming Ma, Yu Zhang\",\"doi\":\"10.1039/d4ay01573h\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis due to their rich content of tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR) based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst catalytic HPRR current amplification strategies for specific identification and highly sensitive detection. Anti-CXCR4 antibody was immobilized on Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bounded to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 107 particles mL-1, with a detection limit of 117 particles mL-1, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1039/d4ay01573h\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01573h","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
HPRR-Based Diatomic Catalyst Electrochemistry Biosensor for Detecting Cancer-Related Extracellular Vesicles
Cancer-associated extracellular vesicles (EVs) are crucial biomarkers for cancer diagnosis due to their rich content of tumor cell information. To efficiently and accurately detect cancer-associated EVs, an electrochemical hydrogen peroxide reduction reaction (HPRR) based biosensor was developed, utilizing enzyme-linked immunosorbent and diatomic catalyst catalytic HPRR current amplification strategies for specific identification and highly sensitive detection. Anti-CXCR4 antibody was immobilized on Au-plated electrode to selectively capture EVs from the sample. Subsequently, Fe/Cu diatomic catalysts, modified with an anti-CD63 antibody, were bounded to the CD63 on the EVs. Quantitative detection of EVs was achieved by measuring the electrical signals from HPRR catalyzed by the labeled Fe/Cu diatomic catalysts. Under optimized conditions, the electrochemical signals exhibited a linear relationship with EV concentration in the range of 500 to 107 particles mL-1, with a detection limit of 117 particles mL-1, maintaining accuracy even in FBS. With its affordability, high sensitivity, and ease of use, this sensor holds significant potential for medication guidance and postoperative evaluation.