阴道加德纳菌、阴道扇贝菌和弧菌在三物种生物膜中强烈影响彼此的转录组

IF 3.3 3区 生物学 Q2 ECOLOGY Microbial Ecology Pub Date : 2024-09-19 DOI:10.1007/s00248-024-02433-9
Lúcia G. V. Sousa, Juliano Novak, Angela França, Christina A. Muzny, Nuno Cerca
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引用次数: 0

摘要

细菌性阴道病(BV)是全球最常见的阴道感染,其特点是阴道上皮细胞上形成多微生物生物膜。虽然加德纳菌属已被证明在 BV 中起着重要作用,但人们对其他菌种如何影响 BV 的发展却知之甚少。因此,我们旨在研究阴道加德纳菌、阴道扇贝菌和弧菌在三物种生物膜中生长时的转录组。在体外形成单种和三种生物膜,提取 RNA 并送去测序。对三物种生物膜进行定量 PCR 分析(qPCR),以评估生物膜的组成。qPCR 结果显示,三物种生物膜主要由 G. vaginalis 组成,P. bivia 是比例最低的物种。RNA 序列分析显示,阴道杆菌、F. vaginae 和 P. bivia 在一起生长时,分别有 432、126 和 39 个差异表达基因。对阴道球菌下调基因进行基因本体富集后,发现了几个与新陈代谢相关的功能,这表明阴道球菌在多微生物生物膜中生长时的新陈代谢活性较低。这项工作强调了生物膜中存在的 3 种不同的 BV 相关细菌会影响彼此的转录组,并让人们深入了解了增强多微生物联合体毒力潜力的分子机制。这些研究结果将有助于了解附带 BV 的发展以及生物膜内发生的相互作用。
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Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia Strongly Influence Each Other's Transcriptome in Triple-Species Biofilms

Bacterial vaginosis (BV), the most common vaginal infection worldwide, is characterized by the development of a polymicrobial biofilm on the vaginal epithelium. While Gardnerella spp. have been shown to have a prominent role in BV, little is known regarding how other species can influence BV development. Thus, we aimed to study the transcriptome of Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia, when growing in triple-species biofilms. Single and triple-species biofilms were formed in vitro, and RNA was extracted and sent for sequencing. cDNA libraries were prepared and sequenced. Quantitative PCR analysis (qPCR) was performed on the triple-species biofilms to evaluate the biofilm composition. The qPCR results revealed that the triple-species biofilms were mainly composed by G. vaginalis and P. bivia was the species with the lowest percentage. The RNA-sequencing analysis revealed a total of 432, 126, and 39 differentially expressed genes for G. vaginalis, F. vaginae, and P. bivia, respectively, when growing together. Gene ontology enrichment of G. vaginalis downregulated genes revealed several functions associated with metabolism, indicating a low metabolic activity of G. vaginalis when growing in polymicrobial biofilms. This work highlighted that the presence of 3 different BV-associated bacteria in the biofilm influenced each other’s transcriptome and provided insight into the molecular mechanisms that enhanced the virulence potential of polymicrobial consortia. These findings will contribute to understand the development of incident BV and the interactions occurring within the biofilm.

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来源期刊
Microbial Ecology
Microbial Ecology 生物-海洋与淡水生物学
CiteScore
6.90
自引率
2.80%
发文量
212
审稿时长
3-8 weeks
期刊介绍: The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.
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