开发和评估用于检测白色念珠菌的环介导等温扩增（LAMP）方法

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2024-09-16 DOI:10.1007/s10096-024-04934-4

H. Yahaya, Y. K. Cheah, H. Y. Chee, L. T. L. Than

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引用次数: 0

摘要

目的环介导等温扩增（LAMP）是一种在恒温条件下进行 DNA 扩增的简单而快速的核酸方法。酵母菌检测的 "金标准 "培养法灵敏度低，后果严重，增加了发病率和死亡率。方法设计并评估了用于检测格氏念珠菌的特异性 LAMP 引物。结果 LAMP 检测能准确检测出格氏念珠菌，且与其他念珠菌种类无交叉反应。

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Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection

Purpose

Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.

Methodology

The specific LAMP primers for C. glabrata detection were designed and evaluated.

Results

The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.

Conclusion

The developed molecular method would be a promising tool in the management of invasive candidiasis.

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.