几内亚比绍比热戈斯群岛梅拉按蚊种群结构和抗杀虫剂标记的全基因组序列分析

IF 3 2区 医学 Q1 PARASITOLOGY Parasites & Vectors Pub Date : 2024-09-18 DOI:10.1186/s13071-024-06476-2
Sophie Moss, Elizabeth Pretorius, Sainey Ceesay, Eunice Teixeira da Silva, Harry Hutchins, Mamadou Ousmane Ndiath, Holly Acford-Palmer, Emma L. Collins, Matthew Higgins, Jody Phelan, Robert T. Jones, Hristina Vasileva, Amabelia Rodrigues, Sanjeev Krishna, Taane G. Clark, Anna Last, Susana Campino
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引用次数: 0

摘要

梅拉按蚊是一种未被充分研究的疟疾病媒,可能在几内亚比绍比热戈斯群岛的疟疾传播中扮演重要角色。本研究首次对比热戈斯群岛的这一物种进行了全基因组测序和种群遗传分析。据我们所知,这也是使用 WGS 数据对非汇集的瓜氏疟蚊进行的最大规模的种群遗传分析。在 2019 年疟疾传播高峰期,我们从比热戈斯群岛的六个不同岛屿上采集了 30 只 An. melas 个体,并对其进行了 WGS 分析。利用生物信息学工具研究了该蚊子种群的种群结构和杀虫剂抗药性标记的流行情况。在比热戈斯群岛的冈比亚按蚊种群中,与拟除虫菊酯抗性相关的杀虫剂抗性突变在梅拉斯按蚊种群中并不存在,在杀虫剂抗性相关基因中也未发现选择性扫描的特征。对结构变异的分析发现了细胞色素-P450基因cyp9k1的大量重复。利用公开的线粒体基因组进行的系统进化分析表明,由于细胞色素 C 氧化酶亚基 COX I 和 COX II 以及 NADH 脱氢酶亚基 1、4、4L 和 5 在线粒体基因组上的分化,来自比热戈斯的黑角蚁分成了两个系统进化组。这项研究发现梅拉斯鳗种群中没有冈比亚鳗常见的抗杀虫剂 SNPs,但确实发现了抗杀虫剂相关基因的结构变异。此外,本研究还利用 WGS 分析对这种疟疾病媒的种群结构提出了新的见解。要进一步了解这种病媒在疟疾传播中的作用,还需要进行更多的研究。
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Whole genome sequence analysis of population structure and insecticide resistance markers in Anopheles melas from the Bijagós Archipelago, Guinea-Bissau
Anopheles melas is an understudied malaria vector with a potential role in malaria transmission on the Bijagós Archipelago of Guinea-Bissau. This study presents the first whole-genome sequencing and population genetic analysis for this species from the Bijagós. To our knowledge, this also represents the largest population genetic analysis using WGS data from non-pooled An. melas mosquitoes. WGS was conducted for 30 individual An. melas collected during the peak malaria transmission season in 2019 from six different islands on the Bijagós Archipelago. Bioinformatics tools were used to investigate the population structure and prevalence of insecticide resistance markers in this mosquito population. Insecticide resistance mutations associated with pyrethroid resistance in Anopheles gambiae s.s. from the Bijagós were absent in the An. melas population, and no signatures of selective sweeps were identified in insecticide resistance-associated genes. Analysis of structural variants identified a large duplication encompassing the cytochrome-P450 gene cyp9k1. Phylogenetic analysis using publicly available mitochondrial genomes indicated that An. melas from the Bijagós split into two phylogenetic groups because of differentiation on the mitochondrial genome attributed to the cytochrome C oxidase subunits COX I and COX II and the NADH dehydrogenase subunits 1, 4, 4L and 5. This study identified an absence of insecticide-resistant SNPs common to An. gambiae in the An. melas population, but did identify structural variation over insecticide resistance-associated genes. Furthermore, this study presents novel insights into the population structure of this malaria vector using WGS analysis. Additional studies are required to further understand the role of this vector in malaria transmission.
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来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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