腔 A MCF-7 乳腺癌细胞中由酸性异磷酸酶驱动的异核苷酸酶活性

IF 3.3 3区 生物学 Q3 CELL BIOLOGY Cell Biology International Pub Date : 2024-09-16 DOI:10.1002/cbin.12237
Marco Antonio Lacerda-Abreu, Bruna dos Santos Mendonça, Gabriela Nestal de Moraes, José Roberto Meyer-Fernandes
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引用次数: 0

摘要

外磷酸酶催化细胞外环境中磷酸化分子(如磷酸氨基酸)的水解。然而,细胞外环境中核苷酸的水解通常是由外切核苷酸酶催化的。研究表明,酸性外切磷酸酶或跨膜前列腺酸性磷酸酶(TM-PAP)(前列腺酸性磷酸酶的膜结合剪接变体)具有外切-5′-核苷酸酶活性。此外,研究还证明,在三阴性乳腺癌细胞中,外切磷酸酶不能水解 ATP、ADP 或 AMP。与之前在 MDA-MB-231 细胞中的发现不同,本研究中研究的外切磷酸酶在腔 A 型乳腺癌细胞(MCF-7)中显示出了水解 AMP 的显著能力。我们发现,AMP 对对硝基苯磷酸(p-NPP)的水解具有剂量依赖性抑制作用。对硝基苯磷酸(p-NPP)和 AMP 的水解表现出相似的生化行为,如在酸性条件下水解增加,氯化镍、钼酸铵和正钒酸钠的抑制作用相当。此外,这种具有外切核苷酸酶活性的外切磷酸酶对于从细胞外微环境中的磷酸化分子中释放腺苷和无机磷酸盐至关重要。这是首次研究表明,乳腺癌细胞(MCF-7)膜表面的前列腺酸性磷酸酶与细胞粘附和迁移有关。
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Ectonucleotidase activity driven by acid ectophosphatase in luminal A MCF-7 breast cancer cells

Ectophosphatases catalyse the hydrolysis of phosphorylated molecules, such as phospho-amino acids, in the extracellular environment. Nevertheless, the hydrolysis of nucleotides in the extracellular environment is typically catalysed by ectonucleotidases. Studies have shown that acid ectophosphatase, or transmembrane-prostatic acid phosphatase (TM-PAP), a membrane-bound splice variant of prostatic acid phosphatase, has ecto-5′-nucleotidase activity. Furthermore, it was demonstrated that ectophosphatase cannot hydrolyse ATP, ADP, or AMP in triple-negative breast cancer cells. In contrast to previous findings in MDA-MB-231 cells, the ectophosphatase studied in the present work displayed a remarkable capacity to hydrolyse AMP in luminal A breast cancer cells (MCF-7). We showed that AMP dose-dependently inhibited p-nitrophenylphosphate (p-NPP) hydrolysis. The p-NPP and AMP hydrolysis showed similar biochemical behaviours, such as increased hydrolysis under acidic conditions and comparable inhibition by NiCl2, ammonium molybdate, and sodium orthovanadate. In addition, this ectophosphatase with ectonucleotidase activity was essential for the release of adenosine and inorganic phosphate from phosphorylated molecules available in the extracellular microenvironment. This is the first study to show that prostatic acid phosphatase on the membrane surface of breast cancer cells (MCF-7) is correlated with cell adhesion and migration.

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来源期刊
Cell Biology International
Cell Biology International 生物-细胞生物学
CiteScore
7.60
自引率
0.00%
发文量
208
审稿时长
1 months
期刊介绍: Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.
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